catalog number :
MBS2012313
products type :
Recombinant Protein
products full name :
Recombinant Kallikrein 1 (KLK1)
products short name :
Kallikrein 1 (KLK1)
other names :
kallikrein-1; Kallikrein-1; kallikrein-1; gamma-NGF; PS kallikrein; pancreatic kallikrein; true tissue kallikrein; nerve growth factor gamma chain; kallikrein 1-related peptidase b3; nerve growth factor gamma polypeptide; kallikrein 1; Gamma-NGF; Nerve growth factor gamma chain; PS kallikrein; Pancreatic kallikrein; RGK-1; rK-1; Tissue kallikrein; True tissue kallikrein
products gene name :
KLK1
other gene names :
Klk1; Ngfg; Ngfg; rK-1; rGK-1; Klk1b3; Klk1c1; Klk-1; Klk1; rK-1
uniprot entry name :
KLK1_RAT
sequence :
The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGS-VQSRVVGGYN CEMNSQPWQV AVYYFGEYLC GGVLIDPSWV ITAAHCATDN YQVWLGRNNL YEDEPFAQHR LVSQSFPHPG FNQDLIWNHT RQPGDDYSND LMLLHLSQPA DITDGVKVID LPIEEPKVGS TCLASGWGSI TPDGLELSDD LQCVNIDLLS NEKCVEAHKE EVTDLMLCAG EMDGGKDTCK GDSGGPLICN GVLQGITSWG FNPCGEPKKP GIYTKLIKFT PWIKEVMKEN P
form :
Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
storage stability :
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). (May be sutiable for use in other assays to be determine by the end user.)
other info1 :
Organism: Rattus norvegicus (Rat). Expression System: Prokaryotic expression. Residues: Val21~Pro261 (Accession # P00758) with N-terminal His-Tag
other info2 :
Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Predicted Isoelectric Point: 4.9. Accurate Molecular Mass: 31kDa as determined by SDS-PAGE reducing conditions.
products description :
About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.
ncbi acc num :
NP_113711.1
ncbi gb acc num :
NM_031523.1
ncbi mol weight :
27.9kDa
ncbi pathways :
Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213301); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213276)
ncbi summary :
a putative pancreatic kallikrein [RGD, Feb 2006]
uniprot summary :
Catalytic activity: Preferential cleavage of Arg- -Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met- -Xaa or Leu- -Xaa. Tissue specificity: High levels in pancreas, submaxillary and parotid glands, spleen, and kidney. Ref.1. Sequence similarities: Belongs to the peptidase S1 family. Kallikrein subfamily.Contains 1 peptidase S1 domain. Sequence caution: The sequence AAA41464.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH78784.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA00346.1 differs from that shown. Reason: Erroneous initiation.