protein

Recombinant Runt Related Transcription Factor 2 (RUNX2)

MBS2012302

0.01 mg

145 USD

Recombinant Protein

Recombinant Runt Related Transcription Factor 2 (RUNX2)

[Runt Related Transcription Factor 2 (RUNX2)]

[runt-related transcription factor 2 isoform b; Runt-related transcription factor 2; runt-related transcription factor 2; PEA2-alpha A; PEBP2-alpha A; oncogene AML-3; acute myeloid leukemia 3 protein; SL3-3 enhancer factor 1 alpha A subunit; osteoblast-specific transcription factor 2; SL3/AKV core-binding factor alpha A subunit; core-binding factor, runt domain, alpha subunit 1; polyomavirus enhancer-binding protein 2 alpha A subunit; runt-related transcription factor 2; Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; CBF-alpha-1; Oncogene AML-3; Osteoblast-specific transcription factor 2; OSF-2; Polyomavirus enhancer-binding protein 2 alpha A subunit; PEA2-alpha A; PEBP2-alpha A; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit]

[RUNX2]

[RUNX2; RUNX2; CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1; AML3; CBFA1; OSF2; PEBP2A; CBF-alpha-1; OSF-2; PEA2-alpha A]

RUNX2_HUMAN

E.coli

Homo sapiens (Human)

> 90%

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Positive Control, Immunogen, SDS-PAGE, WB. (May be suitable for use in other assays to be determined by the end user.)

SDS-Page

Source: Prokaryotic expression. Residues: Tyr299~Tyr521. Tags: N-terminal His Tag. Subcellular Location: Nucleus. Traits: Freeze-dried powder

Predicted isoelectric point: 6.4. Predicted Molecular Mass: 25.3kDa. Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

226442791

NP_001015051.3

NM_001015051.3

Q13950

Androgen Receptor Signaling Pathway (198806); BMP Signalling And Regulation Pathway (198910); Endochondral Ossification Pathway (198812); FGF Signaling Pathway (137989); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Interleukin-11 Signaling Pathway (698753); Notch-mediated HES/HEY Network Pathway (169347); Regulation Of Nuclear SMAD2/3 Signaling Pathway (137963); Regulation Of Retinoblastoma Protein Pathway (137916)

This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2008]

AML3: Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5 - PYGPYGGT-3 , of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation. Interaction with SATB2 results in enhanced DNA binding and transactivation by these transcription factors. Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 and HIPK3. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with KAT6A and KAT6B. Binds to cyclin B1 CCNB1. Interacts with DDX5. Specifically expressed in osteoblasts. 3 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 6p21. Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm. Molecular Function: protein domain specific binding; protein binding; bHLH transcription factor binding; chromatin binding; transcription factor activity; ATP binding. Biological Process: embryonic forelimb morphogenesis; transcription initiation from RNA polymerase II promoter; ossification; positive regulation of transcription, DNA-dependent; cell maturation; regulation of fibroblast growth factor receptor signaling pathway; chondrocyte development; stem cell differentiation; embryonic cranial skeleton morphogenesis; odontogenesis of dentine-containing teeth; osteoblast development; osteoblast differentiation; BMP signaling pathway; positive regulation of osteoblast differentiation; positive regulation of chondrocyte differentiation; negative regulation of smoothened signaling pathway; positive regulation of cell proliferation; gene expression; negative regulation of transcription, DNA-dependent; T cell differentiation; regulation of odontogenesis of dentine-containing teeth; endochondral ossification; osteoblast fate commitment. Disease: Metaphyseal Dysplasia With Maxillary Hypoplasia With Or Without Brachydactyly; Cleidocranial Dysplasia

0.01 mg

145 USD

0.05 mg

285

0.1 mg

445

0.2 mg

545

0.5 mg

1050

1 mg

1565