catalog number :
MBS2012291
products type :
Recombinant Protein
products full name :
Recombinant Metallothionein 2 (MT2)
products short name :
[Metallothionein 2 (MT2)]
other names :
[metallothionein-2; Metallothionein-2; metallothionein-2; MT-2; MT-II; metallothionein-2A; metallothionein-II; metallothionein 2A; Metallothionein-2A; Metallothionein-II]
products gene name :
[MT2]
other gene names :
[MT2A; MT2A; MT2; CES1; MT2; MT-2; MT-II]
uniprot entry name :
MT2_HUMAN
form :
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
concentration :
Original Concentration: 200 Ug/mL
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
tested application :
Positive Control, Immunogen, SDS-PAGE, WB. (May be suitable for use in other assays to be determined by the end user.)
image1 heading :
SDS-Page
other info1 :
Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Asp2~Ala61. Tags: No-Tag. Traits: Freeze-dried powder
. Buffer formulation: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Predicted isoelectric point: 8.3. Predicted Molecular Mass: 5.9kDa. Accurate Molecular Mass: 12kDa as determined by SDS-PAGE reducing conditions.
other info2 :
Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:. 1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc. USAGE: Reconstitute in 100mM NaHCO 3 , 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
products description :
About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.
ncbi acc num :
NP_005944.1
ncbi gb acc num :
NM_005953.3
ncbi pathways :
Cytokine Signaling In Immune System Pathway (366171); Immune System Pathway (106386); Interferon Signaling Pathway (187103); Interferon Gamma Signaling Pathway (187106); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220)
uniprot summary :
MT2A: Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. Belongs to the metallothionein superfamily. Type 1 family. Protein type: Carrier. Chromosomal Location of Human Ortholog: 16q13. Cellular Component: perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus. Molecular Function: protein binding; zinc ion binding; drug binding. Biological Process: cellular copper ion homeostasis; cytokine and chemokine mediated signaling pathway; negative regulation of growth