Recombinant Telomerase Reverse Transcriptase (TERT) | MyBioSource MBS2012286 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Telomerase Reverse Transcriptase (TERT)

catalog :

MBS2012286

quantity :

0.01 mg

price :

195 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2012286

products type :

Recombinant Protein

products full name :

Recombinant Telomerase Reverse Transcriptase (TERT)

products short name :

Telomerase Reverse Transcriptase (TERT)

other names :

telomerase reverse transcriptase; Telomerase reverse transcriptase; telomerase reverse transcriptase; telomerase catalytic subunit; telomerase reverse transcriptase beta; telomerase reverse transcriptase; Telomerase catalytic subunit

products gene name :

TERT

other gene names :

Tert; Tert; TR; TP2; TRT; EST2; TCS1

uniprot entry name :

TERT_MOUSE

host :

E Coli

sequence length :

1122

purity :

> 95%

form :

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), CoIP, ReporterAssays, Purification, Amine Reactive Labeling.

other info1 :

Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Gln642~Lys974 with two N-terminal Tags, His-tag and GST-tag. Subcellular Location: Nucleus. Nucleolus. PML body. Nucleoplasm. Chromosome, telomere. Cytoplasm. Tissue Specificity: Intestine, Liver, Testis, Heart. Traits: Freeze-dried powder

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Predicted isoelectric point: 7.1. Predicted Molecular Mass: 67.4kDa. Accurate Molecular Mass: 60kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:. 1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

ncbi gi num :

6678291

ncbi acc num :

NP_033380.1

ncbi gb acc num :

NM_009354.1

uniprot acc num :

O70372

ncbi pathways :

Cell Cycle Pathway (927078); Chromosome Maintenance Pathway (927161); Extension Of Telomeres Pathway (927165); HTLV-I Infection Pathway (373903); HTLV-I Infection Pathway (373889); IL-2 Signaling Pathway (198331); Id Signaling Pathway (198411); PluriNetWork Pathway (198307); Telomere Extension By Telomerase Pathway (927166); Telomere Maintenance Pathway (927164)

uniprot summary :

TERT: telomerase reverse transcriptase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The holoenzyme consists of TERT and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced isoforms have been identified, although the full-length sequence of some variants has not been determined. Protein type: Transferase; Nucleolus; EC 2.7.7.49; DNA-binding. Cellular Component: nucleoplasm; chromosome, telomeric region; PML body; mitochondrion; telomerase holoenzyme complex; cytoplasm; chromosome; ribonucleoprotein complex; nucleus. Molecular Function: transferase activity; telomerase activity; protein binding; RNA-directed RNA polymerase activity; protein homodimerization activity; DNA binding; metal ion binding; telomeric DNA binding; RNA-directed DNA polymerase activity; telomeric template RNA reverse transcriptase activity; tRNA binding; nucleotidyltransferase activity. Biological Process: mitochondrion organization and biogenesis; DNA strand elongation; positive regulation of hair cycle; RNA-dependent DNA replication; positive regulation of nitric-oxide synthase activity; telomere maintenance via telomerase; age-dependent telomere shortening; RNA interference, production of siRNA; positive regulation of Wnt receptor signaling pathway

size1 :

0.01 mg

price1 :

195 USD

size2 :

0.05 mg

price2 :

415

size3 :

0.1 mg

price3 :

640

more info or order :

MyBioSource product webpage