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Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)

MBS2012165

0.01 mg

250 USD

MBS2012165

Recombinant Protein

Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)

A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)

A disintegrin and metalloproteinase with thrombospondin motifs 1 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 1; A disintegrin and metalloproteinase with thrombospondin motifs 1; METH-1; ADAM-TS1; ADAMTS-1; ADAM-TS 1; human metalloproteinase with thrombospondin type 1 motifs; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1; ADAM metallopeptidase with thrombospondin type 1 motif, 1; METH-1

ADAMTS1

ADAMTS1; ADAMTS1; C3-C5; METH1; KIAA1346; METH1; ADAM-TS 1; ADAM-TS1; ADAMTS-1

ATS1_HUMAN

E Coli

967

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGS-DLPGT SYDANRQCQF TFGEDSKHCP DAASTCSTLW CTGTSGGVLV CQTKHFPWAD GTSCGEGKWC INGKCVNKTD RKHFDTPFHG SWGMWGPWGD CSRTCGGGVQ YTMRECDNPV PKNGGKYCEG KRVRYRSCNL EDCP

> 95%

Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Asp476~Pro614 (Accession # Q9UHI8) with N-terminal His-Tag

Endotoxin Level: Reconstitute in sterile PBS, pH7.2-pH7.4.

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

50845384

NP_008919.3

NM_006988.3

Q9UHI8

16.6kDa

Degradation Of The Extracellular Matrix Pathway 576263!!Degradation Of The Extracellular Matrix Pathway 934113!!Endochondral Ossification Pathway 198812!!Extracellular Matrix Organization Pathway 576262!!Extracellular Matrix Organization Pathway 934100

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function. [provided by RefSeq, Jul 2008]

Function: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover . By similarity. Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture. Ref.2. Catalytic activity: Cleaves aggrecan at the 1938-Glu- -Leu-1939 site, within the chondroitin sulfate attachment domain. Cofactor: Binds 1 zinc ion per subunit. Ref.8. Subcellular location: Secreted extracellular space extracellular matrix . By similarity. Domain: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. Post-translational modification: The precursor is cleaved by a furin endopeptidase . By similarity.Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion . By similarity. Sequence similarities: Contains 1 disintegrin domain.Contains 1 peptidase M12B domain.Contains 3 TSP type-1 domains. Sequence caution: The sequence AAD48080.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA92584.1 differs from that shown. Reason: Erroneous initiation.

0.01 mg

250 USD