Recombinant Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7) | MyBioSource MBS2012154 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)

catalog :

MBS2012154

quantity :

0.01 mg

price :

190 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2012154

products type :

Recombinant Protein

products full name :

Recombinant Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)

products short name :

Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)

other names :

myosin-7; Myosin-7; myosin-7; myHC-beta; myhc-slow; myopathy, distal 1; myosin heavy chain 7; beta-myosin heavy chain; myosin heavy chain (AA 1-96); myosin heavy chain slow isoform; rhabdomyosarcoma antigen MU-RMS-40.7A; myosin heavy chain, cardiac muscle beta isoform; myosin, heavy polypeptide 7, cardiac muscle, beta; myosin, heavy chain 7, cardiac muscle, beta; Myosin heavy chain 7; Myosin heavy chain slow isoform; MyHC-slow; Myosin heavy chain, cardiac muscle beta isoform

products gene name :

MYH7

other gene names :

MYH7; MYH7; CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB; MYHCB; MyHC-slow; MyHC-beta

uniprot entry name :

MYH7_HUMAN

host :

E Coli

sequence length :

1935

sequence :

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF-RSV NDLTSQRAKL QTENGELSRQ LDEKEALISQ LTRGKLTYTQ QLEDLKRQLE EEVKAKNALA HALQSARHDC DLLREQYEEE TEAKAELQRV LSKANSEVAQ WRTKYETDAI QRTEELEEAK KKLAQRLQEA EEAVEAVNAK CSSLEKTKHR LQNEIEDLMV DVERSNAAAA ALDKKQRNFD KILAEWKQKY EESQSELESS QKEARSLSTE LFKLKNAYEE SLEHLETFKR ENKNLQEEIS DLTEQL

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% strehalose, 0.01% sarcosyl, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Arg1268~Leu1516 (Accession # P12883) with N-terminal His-Tag. Predicted isoelectric point: 5.3

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

115496169

ncbi acc num :

NP_000248.2

ncbi gb acc num :

NM_000257.2

uniprot acc num :

P12883

ncbi mol weight :

30.5kDa

ncbi pathways :

Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Cardiac Muscle Contraction Pathway (93344); Cardiac Muscle Contraction Pathway (93992); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Membrane Trafficking Pathway (106160); Tight Junction Pathway (83071)

ncbi summary :

Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy. [provided by RefSeq, Jul 2008]

uniprot summary :

MYH7: Muscle contraction. Defects in MYH7 are the cause of familial hypertrophic cardiomyopathy type 1 (CMH1). Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Defects in MYH7 are the cause of myopathy myosin storage (MYOMS). In this disorder, muscle biopsy shows type 1 fiber predominance and increased interstitial fat and connective tissue. Inclusion bodies consisting of the beta cardiac myosin heavy chain are present in the majority of type 1 fibers, but not in type 2 fibers. Defects in MYH7 are the cause of scapuloperoneal myopathy MYH7-related (SPMM); also known as scapuloperoneal syndrome myopathic type. SPMM is a progressive muscular atrophia beginning in the lower legs and affecting the shoulder region earlier and more severely than distal arm. Defects in MYH7 are a cause of cardiomyopathy dilated type 1S (CMD1S). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in MYH7 are the cause of myopathy distal type 1 (MPD1). MPD1 is a muscular disorder characterized by early-onset selective weakness of the great toe and ankle dorsiflexors, followed by weakness of the finger extensors. Mild proximal weakness occasionally develops years later after the onset of the disease. Protein type: Motility/polarity/chemotaxis; Motor. Chromosomal Location of Human Ortholog: 14q12. Cellular Component: nucleoplasm; sarcomere; focal adhesion; cytoplasm; stress fiber; muscle myosin complex; Z disc; myosin complex. Molecular Function: calmodulin binding; microfilament motor activity; protein binding; ATPase activity; actin binding; actin-dependent ATPase activity; ATP binding. Biological Process: adult heart development; striated muscle contraction; muscle contraction; regulation of heart rate; metabolic process; ventricular cardiac muscle morphogenesis; regulation of the force of heart contraction; muscle filament sliding. Disease: Myopathy, Distal, 1; Scapuloperoneal Myopathy, Myh7-related; Myopathy, Myosin Storage; Cardiomyopathy, Dilated, 1s; Cardiomyopathy, Familial Hypertrophic, 1; Myopathy, Congenital, With Fiber-type Disproportion; Myopathy, Myosin Storage, Autosomal Recessive

size1 :

0.01 mg

price1 :

190 USD

size2 :

0.05 mg

price2 :

395

size3 :

0.1 mg

price3 :

610

more info or order :

MyBioSource product webpage