Recombinant Solute Carrier Family 30 Member 8 (SLC30A8) | MyBioSource MBS2012153 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Solute Carrier Family 30 Member 8 (SLC30A8)

catalog :

MBS2012153

quantity :

0.01 mg

price :

290 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2012153

products type :

Recombinant Protein

products full name :

Recombinant Solute Carrier Family 30 Member 8 (SLC30A8)

products short name :

Solute Carrier Family 30 Member 8 (SLC30A8)

other names :

zinc transporter 8 isoform b; Zinc transporter 8; zinc transporter 8; zinc transporter ZnT-8; solute carrier family 30 (zinc transporter), member 8; Solute carrier family 30 member 8

products gene name :

SLC30A8

other gene names :

SLC30A8; SLC30A8; ZNT8; ZnT-8; ZNT8; ZnT-8

uniprot entry name :

ZNT8_HUMAN

host :

E Coli

sequence length :

320

sequence :

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-TITILKDF SILLMEGVPK SLNYSGVKEL ILAVDGVLSV HSLHIWSLTM NQVILSAHVA TAASRDSQVV RREIAKALSK SFTMHSLTIQ MESPVDQDPD CLFCEDPCD

purity :

>95%

form :

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), CoIP, ReporterAssays, Purification, Amine Reactive Labeling.

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Thr263~Asp369 with N-terminal His-Tag. Subcellular Location: Cell membrane; Cytoplasmic vesicle, secretory vesicle membrane; Multi-pass membrane protein. Tissue Specificity: Brain, Kidney, Pancreas, Lung.

other info2 :

Predicted Isoelectric Point: 5.9. Predicted Molecular Mass: 13.3kDa. Accurate Molecular Mass: 15kDa as determined by SDS-PAGE reducing conditions. Traits: Freeze-dried powder . Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

289803003

ncbi acc num :

NP_001166282.1

ncbi gb acc num :

NM_001172811.1

uniprot acc num :

Q8IWU4

ncbi mol weight :

13.3kDa

ncbi pathways :

Metal Ion SLC Transporters Pathway (161066); SLC-mediated Transmembrane Transport Pathway (119558); Transmembrane Transport Of Small Molecules Pathway (106572); Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway (119571); Zinc Efflux And Compartmentalization By The SLC30 Family Pathway (161068); Zinc Transporters Pathway (161067)

ncbi summary :

The protein encoded by this gene is a zinc efflux transporter involved in the accumulation of zinc in intracellular vesicles. This gene is expressed at a high level only in the pancreas, particularly in islets of Langerhans. The encoded protein colocalizes with insulin in the secretory pathway granules of the insulin-secreting INS-1 cells. Allelic variants of this gene exist that confer susceptibility to diabetes mellitus, noninsulin-dependent (NIDDM). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]

uniprot summary :

SLC30A8: Facilitates the accumulation of zinc from the cytoplasm into intracellular vesicles, being a zinc-efflux transporter. May be a major component for providing zinc to insulin maturation and/or storage processes in insulin-secreting pancreatic beta- cells. Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transporter; Membrane protein, integral; Vesicle; Membrane protein, multi-pass; Transporter, SLC family. Chromosomal Location of Human Ortholog: 8q24.11. Cellular Component: Golgi apparatus; secretory granule membrane; cytoplasmic membrane-bound vesicle; plasma membrane; integral to membrane; secretory granule. Molecular Function: protein homodimerization activity; zinc ion binding; zinc ion transmembrane transporter activity. Biological Process: sequestering of zinc ion; cellular protein metabolic process; insulin secretion; cellular zinc ion homeostasis; response to glucose stimulus; positive regulation of insulin secretion; zinc ion transport; glucose homeostasis; transmembrane transport. Disease: Diabetes Mellitus, Noninsulin-dependent

size1 :

0.01 mg

price1 :

290 USD

size2 :

0.05 mg

price2 :

690

size3 :

0.1 mg

price3 :

1080

more info or order :

MyBioSource product webpage