Recombinant Islet Cell Autoantigen 1 (ICA1) | MyBioSource MBS2012102 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Islet Cell Autoantigen 1 (ICA1)

catalog :

MBS2012102

quantity :

0.01 mg

price :

200 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2012102

products type :

Recombinant Protein

products full name :

Recombinant Islet Cell Autoantigen 1 (ICA1)

products short name :

Islet Cell Autoantigen 1 (ICA1)

other names :

islet cell autoantigen 1 isoform a; Islet cell autoantigen 1; islet cell autoantigen 1; p69; islet cell autoantigen p69; 69 kDa islet cell autoantigen; islet cell autoantigen 1 isoform; diabetes mellitus type I autoantigen; islet cell autoantigen 1, 69kDa; 69 kDa islet cell autoantigen; ICA69; Islet cell autoantigen p69

products gene name :

ICA1

other gene names :

ICA1; ICA1; ICA69; ICAp69; ICA69; ICAp69; p69

uniprot entry name :

ICA69_HUMAN

host :

E. coli

sequence length :

483

sequence :

The sequence of the target protein is listed below. MSGHKCSYPW DLQDRYAQDK SVVNKMQQKY WETKQAFIKA TGKKEDEHVV ASDADLDAKL ELFHSIQRTC LDLSKAIVLY QKRICFLSQE ENELGKFLRS QGFQDKTRAG KMMQATGKAL CFSSQQRLAL RNPLCRFHQE VETFRHRAIS DTWLTVNRME QCRTEYRGAL LWMKDVSQEL DPDLYKQMEK FRKVQTQVRL AKKNFDKLKM DVCQKVDLLG ASRCNLLSHM LATYQTTLLH FWEKTSHTMA AIHESFKG

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

other info1 :

Source: Prokaryotic expression. Residues: Met1~Gly258. Tags: N-terminal His-Tag. Tissue Specificity: Brain. Subcellular Location: Cytoplasm, cytosol. Golgi apparatus membrane; Cytoplasmic vesicle, secretory vesicle membrane; secretory vesicle. Peripheral membrane protein. Traits: Freeze-dried powder

other info2 :

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Predicted isoelectric point: 8.8. Predicted Molecular Mass: 36.9kDa. Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:. 1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc.

ncbi gi num :

209862841

ncbi acc num :

NP_001129492.1

ncbi gb acc num :

NM_001136020.2

uniprot acc num :

Q05084

ncbi pathways :

Type I Diabetes Mellitus Pathway (83095); Type I Diabetes Mellitus Pathway (507)

ncbi summary :

This gene encodes a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. This protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogren's syndrome. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]

uniprot summary :

ICA1: May play a role in neurotransmitter secretion. Protein type: Unknown function. Chromosomal Location of Human Ortholog: 7p22. Cellular Component: Golgi membrane; synaptic vesicle membrane; secretory granule membrane; cytoplasm; cytosol; cell junction. Molecular Function: protein domain specific binding. Biological Process: neurotransmitter transport

size1 :

0.01 mg

price1 :

200 USD

size2 :

0.05 mg

price2 :

430

size3 :

0.1 mg

price3 :

665

more info or order :

MyBioSource product webpage