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Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)

MBS2012084

0.01 mg

265 USD

MBS2012084

Recombinant Protein

Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)

A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)

A disintegrin and metalloproteinase with thrombospondin motifs 1; A disintegrin and metalloproteinase with thrombospondin motifs 1; A disintegrin and metalloproteinase with thrombospondin motifs 1; ADAM-TS1; ADAMTS-1; ADAM-TS 1; a disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS-1); a disintegrin-like and metallopeptidse (reprolysin type) with thrombospondin type 1 motif, 1; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1; a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 1; ADAM metallopeptidase with thrombospondin type 1 motif, 1; N/A

ADAMTS1

Adamts1; Adamts1; ADAM-TS 1; ADAM-TS1; ADAMTS-1

ATS1_RAT

E Coli

967

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-TFSEWVI EEWGECSKTC GSGWQRRVVE CRDINGHPAS ECAKEVKPAS TRPCADLPCP RWQVGDWSPC SKTCGKGYKK RTLKCLSHDG GVLSNESCDP LKKPKHYIDF CILTQCS

> 95%

Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Organism: Rattus norvegicus (Rat). Expression System: Prokaryotic expression. Residues: Thr854~Ser967 (Accession # Q9WUQ1) with N-terminal His-Tag

Endotoxin Level: Reconstitute in sterile PBS, pH7.2-pH7.4.

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

11131014

Q9WUQ1.1

Q9WUQ1

14.3kDa

Endochondral Ossification Pathway 219771

disintegrin and metalloprotease that may be necessary for normal kidney morphology and function [RGD, Feb 2006]

Function: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. Has angiogenic inhibitor activity . By similarity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture . By similarity. Catalytic activity: Cleaves aggrecan at the 1683-Glu- -Leu-1684 site, within the chondroitin sulfate attachment domain. Cofactor: Binds 1 zinc ion per subunit . By similarity. Subcellular location: Secreted extracellular space extracellular matrix . By similarity. Induction: Down-regulated in endothelial cells derived from cirrhotic liver. Domain: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. Post-translational modification: The precursor is cleaved by a furin endopeptidase . By similarity.Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion . By similarity. Sequence similarities: Contains 1 disintegrin domain.Contains 1 peptidase M12B domain.Contains 3 TSP type-1 domains.

0.01 mg

265 USD