Recombinant Leucine Rich Repeat Kinase 2 (LRRK2) | MyBioSource MBS2012043 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Leucine Rich Repeat Kinase 2 (LRRK2)

catalog :

MBS2012043

quantity :

0.01 mg

price :

200 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2012043

products type :

Recombinant Protein

products full name :

Recombinant Leucine Rich Repeat Kinase 2 (LRRK2)

products short name :

Leucine Rich Repeat Kinase 2 (LRRK2)

other names :

leucine-rich repeat serine/threonine-protein kinase 2; Leucine-rich repeat serine/threonine-protein kinase 2; leucine-rich repeat serine/threonine-protein kinase 2; augmented in rheumatoid arthritis 17; leucine-rich repeat kinase 2; Dardarin

products gene name :

LRRK2

other gene names :

LRRK2; LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN; PARK8

uniprot entry name :

LRRK2_HUMAN

host :

E Coli

sequence length :

2527

sequence :

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-FP AIRDYHFVNA TEESDALAKL RKTIINESLN FKIRDQLVVG QLIPDCYVEL EKIILSERKN VPIEFPVIDR KRLLQLVREN QLQLDENELP HAVHFLNESG VLLHFQDPAL QLSDLYFVEP KWLCKIMAQI LTVKVEGCPK HPKGIISRRD VEKFLSKKRK FPKNYMSQYF KLLEKFQIAL PIGEEYLLVP SSLSDHRPVI ELPHCENSEI IIRLYEMPYF PMGFWSRLIN RLLEISPYML SGRERALRP

purity :

> 95%

form :

Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Homo sapiens (Human). Subcellular Location: Cytoplasm. Membrane;Peripheral membrane protein. Mitochondrion.Residues: Phe1479~Pro1729 (Accession # Q5S007) with N-terminal His-Tag

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile ddH2O. Predicted Isoelectric Point: 7.8

ncbi gi num :

171846278

ncbi acc num :

NP_940980.3

ncbi gb acc num :

NM_198578.3

uniprot acc num :

Q5S007

ncbi mol weight :

31.0kDa

ncbi pathways :

MAPK Signaling Pathway (198779); Parkinson's Disease Pathway (83098); Parkinsons Disease Pathway (705377); Wnt Signaling Pathway And Pluripotency (198847)

ncbi summary :

This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq, Jul 2008]

uniprot summary :

LRRK2: a large multidomain protein kinase with a TKL-type kinase domain, multiple protein-protein interaction domains and a mitochondrial Rho domain (MIRO). May play a role in the etiology of Parkinson disease. May also have GTPase activity. Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Interacts with PARK2, PRDX3 and TPCN2. Expressed throughout the adult brain, but at a lower level than in heart and liver. Expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas. Defects in LRRK2 are the cause of Parkinson disease type 8 (PARK8). A slowly progressive neurodegenerative disorder characterized by bradykinesia, rigidity, resting tremor, postural instability, neuronal loss in the substantia nigra, and the presence of neurofibrillary MAPT (tau)-positive and Lewy bodies in some patients. Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; TKL group; LRRK family. Chromosomal Location of Human Ortholog: 12q12. Cellular Component: dendrite cytoplasm; Golgi apparatus; extracellular space; mitochondrion; lysosome; dendrite; terminal button; inclusion body; trans-Golgi network; cytosol; lipid raft; mitochondrial outer membrane; cell soma; mitochondrial matrix; mitochondrial inner membrane; cytoplasm; cytoplasmic vesicle; endosome; neuron projection; synaptic vesicle membrane; endoplasmic reticulum; perikaryon; axon; mitochondrial membrane; plasma membrane; cell junction. Molecular Function: tubulin binding; GTPase activity; identical protein binding; GTP-dependent protein kinase activity; clathrin binding; protein homodimerization activity; Rho GTPase binding; GTP binding; actin binding; GTPase activator activity; protein kinase activity; MAP kinase kinase activity; protein serine/threonine kinase activity; SNARE binding; protein binding; protein kinase A binding; syntaxin-1 binding; kinase activity; glycoprotein binding; ATP binding. Biological Process: regulation of synaptic transmission, glutamatergic; positive regulation of protein binding; activation of MAPKK activity; activation of MAPK activity; protein amino acid autophosphorylation; regulation of neuron maturation; determination of adult life span; protein amino acid phosphorylation; positive regulation of MAP kinase activity; tangential migration from the subventricular zone to the olfactory bulb; negative regulation of protein amino acid phosphorylation; regulation of dopamine receptor signaling pathway; small GTPase mediated signal transduction; regulation of mitochondrial depolarization; negative regulation of protein binding; neuromuscular junction development; positive regulation of autophagy; positive regulation of dopamine receptor signaling pathway; olfactory bulb development; MAPKKK cascade; peptidyl-threonine phosphorylation; endocytosis; regulation of locomotion; peptidyl-serine phosphorylation; positive regulation of programmed cell death; regulation of membrane potential; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; intracellular distribution of mitochondria; autophagy; positive regulation of protein amino acid phosphorylation; response to oxidative stress; regulation of excitatory postsynaptic membrane potential; neurite morphogenesis; positive regulation of GTPase activity. Disease: Parkinson Disease 8, Autosomal Dominant

size1 :

0.01 mg

price1 :

200 USD

size2 :

0.05 mg

price2 :

430

size3 :

0.1 mg

price3 :

665

more info or order :

MyBioSource product webpage