Recombinant Troponin I Type 1, Slow Skeletal (TNNI1) | MyBioSource MBS2012003 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Troponin I Type 1, Slow Skeletal (TNNI1)

catalog :

MBS2012003

quantity :

0.01 mg

price :

170 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2012003

products type :

Recombinant Protein

products full name :

Recombinant Troponin I Type 1, Slow Skeletal (TNNI1)

products short name :

Troponin I Type 1, Slow Skeletal (TNNI1)

other names :

troponin I, slow skeletal muscle; Troponin I, slow skeletal muscle; troponin I, slow skeletal muscle; troponin I, slow-twitch isoform; troponin I type 1 (skeletal, slow); Troponin I, slow-twitch isoform

products gene name :

TNNI1

other gene names :

TNNI1; TNNI1; TNN1; SSTNI

uniprot entry name :

TNNI1_HUMAN

host :

E Coli

sequence length :

187

sequence :

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-MPEVERKPKI TASRKLLLKS LMLAKAKECW EQEHEEREAE KVRYLAERIP TLQTRGLSLS ALQDLCRELH AKVEVVDEER YDIEAKCLHN TREIKDLKLK VMDLRGKFKR PPLRRVRVSA DAMLRALLGS KHKVSMDLRA NLKSVKKEDT EKERPVEVGD WRKNVEAMSG MEGRKKMFDA AKSPTSQ

purity :

> 95%

form :

Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Met1~Gln187 (Accession # P19237) with N-terminal His-Tag. Predicted isoelectric point: 9.5

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

56682969

ncbi acc num :

NP_003272.3

ncbi gb acc num :

NM_003281.3

uniprot acc num :

P19237

ncbi mol weight :

23.2kDa

ncbi pathways :

Muscle Contraction Pathway (106261); Striated Muscle Contraction Pathway (198903); Striated Muscle Contraction Pathway (106262)

ncbi summary :

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. [provided by RefSeq, Jul 2008]

uniprot summary :

TNNI1: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Belongs to the troponin I family. Protein type: Motility/polarity/chemotaxis; Cytoskeletal. Chromosomal Location of Human Ortholog: 1q31.3. Cellular Component: troponin complex; cytosol. Molecular Function: metal ion binding; actin binding. Biological Process: regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; muscle filament sliding

size1 :

0.01 mg

price1 :

170 USD

size2 :

0.05 mg

price2 :

345

size3 :

0.1 mg

price3 :

535

more info or order :

MyBioSource product webpage