Recombinant Troponin T Type 1, Slow Skeletal (TNNT1) | MyBioSource MBS2011979 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Troponin T Type 1, Slow Skeletal (TNNT1)

catalog :

MBS2011979

quantity :

0.01 mg

price :

135 USD

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

Figure. SDS-PAGE

image 3 :

Figure. Gene Sequencing (Extract)

product information

catalog number :

MBS2011979

products type :

Recombinant Protein

products full name :

Recombinant Troponin T Type 1, Slow Skeletal (TNNT1)

products short name :

[Troponin T Type 1, Slow Skeletal (TNNT1)]

other names :

[troponin T, slow skeletal muscle isoform b; Troponin T, slow skeletal muscle; troponin T, slow skeletal muscle; nemaline myopathy type 5; troponin-T1, skeletal, slow; slow skeletal muscle troponin T; troponin T type 1 (skeletal, slow); Slow skeletal muscle troponin T]

products gene name :

[TNNT1]

other gene names :

[TNNT1; TNNT1; ANM; TNT; NEM5; STNT; TNTS; TNT; TnTs; sTnT]

uniprot entry name :

TNNT1_HUMAN

host :

E. coli

purity :

> 90%

form :

PBS, pH7.4, containing 0.01% SKL.

concentration :

Original Concentration: 200ug/mL

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Positive Control; Immunogen; SDS-PAGE; Western Blot (WB)

app notes :

(May be suitable for use in other assays to be determined by the end user.)

image1 heading :

Sequence Information

image2 heading :

SDS-Page

image3 heading :

Testing Data

other info1 :

Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Met1~Gly259. Tags: N-terminal His Tag. Traits: Freeze-dried powder. Predicted isoelectric point: 5.7. Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:. 1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. Usage: Reconstitute in PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

187173290

ncbi acc num :

NP_001119604.1

ncbi gb acc num :

NM_001126132.1

uniprot acc num :

P13805

ncbi mol weight :

Predicted: 34.2kDa. Accurate: 40kDa as determined by SDS-PAGE reducing conditions.

ncbi pathways :

Muscle Contraction Pathway (106261); Striated Muscle Contraction Pathway (198903); Striated Muscle Contraction Pathway (106262)

ncbi summary :

This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

TNNT1: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNT1 are the cause of nemaline myopathy type 5 (NEM5); also known as Amish nemaline myopathy (ANM). A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 5 is a severe and progressive form common among Old Order Amish. Affected infants display tremors with hypotonia and mild contractures of the shoulders and hips. Proximal contractures progressively weaken and a pectus carinatum deformity develops before children die of respiratory insufficiency, usually in the second year. Belongs to the troponin T family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Contractile. Chromosomal Location of Human Ortholog: 19q13.4. Cellular Component: troponin complex; cytosol. Molecular Function: troponin T binding; tropomyosin binding. Biological Process: skeletal muscle contraction; slow-twitch skeletal muscle fiber contraction; negative regulation of muscle contraction; muscle filament sliding. Disease: Nemaline Myopathy 5

size1 :

0.01 mg

price1 :

135 USD

size2 :

0.05 mg

price2 :

250

size3 :

0.1 mg

price3 :

385

size4 :

0.2 mg

price4 :

470

size5 :

0.5 mg

price5 :

910

size6 :

1 mg

price6 :

1345

more info or order :

MyBioSource product webpage