Recombinant Apolipoprotein A5 (APOA5) | MyBioSource MBS2011902 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Apolipoprotein A5 (APOA5)

catalog :

MBS2011902

quantity :

0.01 mg

price :

200 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2011902

products type :

Recombinant Protein

products full name :

Recombinant Apolipoprotein A5 (APOA5)

products short name :

Apolipoprotein A5 (APOA5)

other names :

apolipoprotein A-V; Apolipoprotein A-V; apolipoprotein A-V; apolipoprotein A5; regeneration-associated protein 3; apolipoprotein A-V; Apolipoprotein A5; Regeneration-associated protein 3

products gene name :

APOA5

other gene names :

Apoa5; Apoa5; apo-AV; apoA-V; Rap3; Apo-AV; ApoA-V

uniprot entry name :

APOA5_RAT

host :

E Coli

reactivity :

Rattus norvegicus (Rat)

sequence length :

367

sequence :

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-QD MQSRVLHHTD RVKELFHPYA ERLVTGIGHH VQELHRSVAP HAVASPARLS RCVQTLSHKL TRKAKDLHTS IQRNLDQLRD ELSTFIRVST DGADNRDSLD PQALSDEVRQ RLQAFRHDTY LQIAAFTQAI DQETEEIQHQ LAPPPPSHSA FAPELGHSDS NKALSRLQSR LDDLWEDIAY GLHDQGHSQN NPEGHSG

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Expression System: Prokaryotic expression. Residues: Gln169~Gly367 (Accession # Q9QUH3) with N-terminal His-Tag. Predicted isoelectric point: 6.2. Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions.

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in ddH2O.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

471270246

ncbi acc num :

NP_001264193.1

ncbi gb acc num :

NM_001277264.1

uniprot acc num :

Q9QUH3

ncbi mol weight :

24.0kDa

ncbi pathways :

Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (935489); Metabolism Pathway (935443); Metabolism Of Lipids And Lipoproteins Pathway (935480); PPAR Signaling Pathway (83434); PPAR Signaling Pathway (450); PPARA Activates Gene Expression Pathway (935509); Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway (935508)

ncbi summary :

member of the apolipoprotein family; expression increases during liver regeneration [RGD, Feb 2006]

uniprot summary :

APOA5: Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Interacts with GPIHBP1. Up-regulated by PPARA agonists, which are used clinically to lower serum TG (such as fibrates). Liver and plasma. Belongs to the apolipoprotein A1/A4/E family. Protein type: Lipid-binding; Secreted, signal peptide; Secreted. Cellular Component: extracellular space; chylomicron; extracellular region. Molecular Function: heparin binding; enzyme binding; low-density lipoprotein receptor binding; cholesterol transporter activity; enzyme activator activity; cholesterol binding; phospholipid binding; phosphatidylcholine binding; lipid binding. Biological Process: positive regulation of fatty acid biosynthetic process; organ regeneration; response to hormone stimulus; neurite regeneration; positive regulation of lipid catabolic process; cholesterol efflux; lipoprotein metabolic process; lipid transport; cholesterol biosynthetic process; phosphatidylcholine metabolic process; cholesterol homeostasis; triacylglycerol metabolic process; reverse cholesterol transport; phospholipid efflux; regulation of cholesterol absorption; acylglycerol homeostasis; tissue regeneration; triacylglycerol catabolic process; positive regulation of lipoprotein lipase activity

size1 :

0.01 mg

price1 :

200 USD

size2 :

0.05 mg

price2 :

435

size3 :

0.1 mg

price3 :

675

more info or order :

MyBioSource product webpage