Recombinant Receptor Interacting Serine Threonine Kinase 3 (RIPK3) | MyBioSource MBS2011887 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Receptor Interacting Serine Threonine Kinase 3 (RIPK3)

catalog :

MBS2011887

quantity :

0.01 mg

price :

190 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2011887

products type :

Recombinant Protein

products full name :

Recombinant Receptor Interacting Serine Threonine Kinase 3 (RIPK3)

products short name :

Receptor Interacting Serine Threonine Kinase 3 (RIPK3)

other names :

receptor-interacting serine/threonine-protein kinase 3; Receptor-interacting serine/threonine-protein kinase 3; receptor-interacting serine/threonine-protein kinase 3; RIP-3; RIP-like protein kinase 3; receptor-interacting protein 3; homocysteine respondent protein HCYP2; receptor-interacting serine-threonine kinase 3; Homocysteine respondent protein HCYP2; RIP-like protein kinase 3; Receptor-interacting protein 3

products gene name :

RIPK3

other gene names :

Ripk3; Ripk3; Hcyp2; Rip3; RIP-3

uniprot entry name :

RIPK3_RAT

host :

E Coli

sequence length :

478

sequence :

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- V KIVNSKKISR EVKAMVNLRH ENVLLLLGVT ENLEWDYVYG PALVTGFMEN GSLSGLLQPS CPRPWPLLCR LLEEVVLGMC YLHSLNPSLL HRDLKPSNVL LDLELHAKLA DFGLSTFQGG SQSGSGSGSR DSGGTLAYLA PELLDNDGKA SKASDVYSFG VLVWTVLAGR EAEVVDKTSL IRGAVCNRQR RPPLTELPPD SPETPGLEGL KELMTHCWSS EP

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Rattus norvegicus (Rat). Expression System: Prokaryotic expression. Residues: Val50~Pro272 (Accession # Q9Z2P5) with two N-terminal Tags, His-tag and T7-tag. Usage: Reconstitute in ddH2O.

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Predicted isoelectric point: 6.3

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

21326477

ncbi acc num :

NP_647558.1

ncbi gb acc num :

NM_139342.1

uniprot acc num :

Q9Z2P5

ncbi mol weight :

28.1kDa

ncbi pathways :

Activated TLR4 Signalling Pathway (936709); Cytosolic DNA-sensing Pathway (125115); Cytosolic DNA-sensing Pathway (124833); Cytosolic Sensors Of Pathogen-associated DNA Pathway (936738); IKK Complex Recruitment Mediated By RIP1 Pathway (936696); Immune System Pathway (936650); Innate Immune System Pathway (936679); MyD88-independent Cascade Pathway (936693); RIP-mediated NFkB Activation Via ZBP1 Pathway (936741); TNF Signaling Pathway (812267)

ncbi summary :

a putative homocysteine respondent protein [RGD, Feb 2006]

uniprot summary :

RIPK3: Promotes apoptosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Binds TRAF2 and RIPK1 and is recruited to the TNFR-1 signaling complex. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, TKL; TKL group; RIPK family. Cellular Component: mitochondrion; plasma membrane; intracellular; cytosol. Molecular Function: NF-kappaB-inducing kinase activity; protein serine/threonine kinase activity; identical protein binding; protein complex binding; ATP binding; protein kinase activity. Biological Process: spleen development; regulation of activated T cell proliferation; I-kappaB kinase/NF-kappaB cascade; thymus development; protein heterooligomerization; protein amino acid autophosphorylation; T cell differentiation in the thymus; positive regulation of transferase activity; protein amino acid phosphorylation; lymph node development; activation of NF-kappaB transcription factor; regulation of T cell mediated cytotoxicity; regulation of adaptive immune response; regulation of interferon-gamma production; activation of protein kinase activity; T cell homeostasis; positive regulation of ligase activity; protein homooligomerization; positive regulation of oxidoreductase activity

size1 :

0.01 mg

price1 :

190 USD

size2 :

0.05 mg

price2 :

410

size3 :

0.1 mg

price3 :

630

more info or order :

MyBioSource product webpage