catalog number :
MBS2011856
products type :
Recombinant Protein
products full name :
Recombinant Colony Stimulating Factor 3, Granulocyte (GCSF)
products short name :
Colony Stimulating Factor 3, Granulocyte (GCSF)
other names :
granulocyte colony-stimulating factor; Granulocyte colony-stimulating factor; granulocyte colony-stimulating factor
products gene name :
GCSF
other gene names :
CSF3; CSF3; G-CSF; G-CSF
uniprot entry name :
CSF3_PIG
sequence :
The target protein is fused with N-terminal His-Tag, its sequence is listed below. FLLKCLE QVRKIQADGA ELQERLCATH KLCHPQELVL LGHSLGLPQA SLSSCSSQAL
QLTGCLNQLH GGLVLYQGLL QALAGISPEL APALDILQLD VTDLATNIWL QMEDLRMAPA
SLPTQGTVPT FTSAFQRRAG GVLVVSQLQSFLELAYRVLR YLA
form :
Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.05% sarcosyl and preservative.
storage stability :
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
other info1 :
Organism: Sus scrofa; Porcine (Pig). Expression System: Prokaryotic expression. Residues: Phe34~Ala193 (Accession # O02837) with N-terminal His-Tag. Subcellular Location: Secreted
other info2 :
Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Predicted Isoelectric Point: 6.3. Reconstitution: Reconstitute in sterile ddH2O
ncbi acc num :
NP_999007.1
ncbi gb acc num :
NM_213842.1
ncbi mol weight :
18.9kDa
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (84463); Cytokine-cytokine Receptor Interaction Pathway (460); Hematopoietic Cell Lineage Pathway (84490); Hematopoietic Cell Lineage Pathway (489); Jak-STAT Signaling Pathway (84489); Jak-STAT Signaling Pathway (488); Malaria Pathway (152663); Malaria Pathway (152657); PI3K-Akt Signaling Pathway (692249); PI3K-Akt Signaling Pathway (692979)
uniprot summary :
Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes . By similarity. Subunit structure: Monomer. Subcellular location: Secreted. Post-translational modification: O-glycosylated . By similarity. Sequence similarities: Belongs to the IL-6 superfamily.