Recombinant Mucin 1 (MUC1) | MyBioSource MBS2011739 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Mucin 1 (MUC1)

catalog :

MBS2011739

quantity :

0.01 mg

price :

190 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2011739

products type :

Recombinant Protein

products full name :

Recombinant Mucin 1 (MUC1)

products short name :

Mucin 1 (MUC1)

other names :

mucin-1; Mucin-1; mucin-1; episialin; mucin 1, transmembrane; Episialin; CD_antigen: CD227Cleaved into the following 2 chains:Mucin-1 subunit alpha; MUC1-NT; MUC1-alpha; Mucin-1 subunit beta; MUC1-betaAlternative name(s):MUC1-CT

products gene name :

MUC1

other gene names :

Muc1; Muc1; EMA; CD227; Muc-1; Muc-1; MUC-1; MUC1-NT; MUC1-alpha; MUC1-beta

uniprot entry name :

MUC1_MOUSE

host :

E Coli

reactivity :

Mus musculus (Mouse)

sequence length :

631

sequence :

The sequence of the target protein is listed below. S PQLSVGVSFF FLSFYIQNHP FNSSLEDPSS NYYQELKRNI SGLFLQIFNG DFLGISSIKF RSGSVVVEST VVFREGTFSA SDVKSQLIQH KKEADDYNLT ISEVKVNEMQ FPPSA

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Expression System: Prokaryotic expression. Residues: Ser410~Ala525 (Accession # Q02496) with N-terminal His-Tag

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in ddH2O. Subcellular Location: Apical cell membrane; Single-pass type I membrane protein. Cell membrane. Cytoplasm. Nucleus. Predicted isoelectric point: 6.0. Accurate Molecular Mass: 10kDa as determined by SDS-PAGE reducing conditions.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

7305293

ncbi acc num :

NP_038633.1

ncbi gb acc num :

NM_013605.2

uniprot acc num :

Q02496

ncbi mol weight :

14.6kDa

ncbi pathways :

IL-7 Signaling Pathway (198324); Metabolism Of Proteins Pathway (926589); O-linked Glycosylation Of Mucins Pathway (926644); Post-translational Protein Modification Pathway (926613); T Cell Receptor Signaling Pathway (198362); Termination Of O-glycan Biosynthesis Pathway (926645)

uniprot summary :

MUC1: a large cell surface glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. Plays a role in adhesion and cell-cell interactions, metastasis and signaling. May provide a protective layer on epithelial surfaces. Direct or indirect interaction with actin cytoskeleton. Its cytoplasmic tail (MUC1CT) is involved in several signaling pathways, including those involving Ras, beta-catenin, p120 catenin, p53 and estrogen receptor alpha. MUC1CT also forms complexes with transcription factors, and then translocates to the nucleus by an unknown mechanism, where it is believed to influence the transcription of their target genes. MUC1CT has also been proposed to localize to mitochondrial membranes under conditions of genotoxic stress, where it attenuates the apoptotic pathway in response and confers resistance to apoptosis-inducing drugs. Aberrantly glycosylated forms expressed in human epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Nine alternatively spliced isoforms have been described. Isoforms 5 and 9 are secreted. Isoform 7, expressed only in tumor cells, is a receptor and binds the secreted isoform 5. The binding induces the phosphorylation of the isoform 7, alters cellular morphology and initiates cell signaling. Can bind to GRB2 adapter protein. Protein type: Cell adhesion; Nuclear receptor co-regulator; Tumor suppressor; Membrane protein, integral; Actin-binding; Motility/polarity/chemotaxis. Cellular Component: cell surface; membrane; apical plasma membrane; cytoplasm; integral to membrane; plasma membrane; nucleus

size1 :

0.01 mg

price1 :

190 USD

size2 :

0.05 mg

price2 :

405

size3 :

0.1 mg

price3 :

625

more info or order :

MyBioSource product webpage