Recombinant Complement Component 3a (C3a) | MyBioSource MBS2011715 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Complement Component 3a (C3a)

catalog :

MBS2011715

quantity :

0.01 mg

price :

185 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2011715

products type :

Recombinant Protein

products full name :

Recombinant Complement Component 3a (C3a)

products short name :

Complement Component 3a (C3a)

other names :

complement C3; Complement C3; complement C3; complement factor 3; complement component 3d; acylation stimulating protein; complement component 3; HSE-MSFCleaved into the following 11 chains:Complement C3 beta chainComplement C3 alpha chainC3a anaphylatoxinAcylation stimulating protein; ASP; Alternative name(s):; C3adesArg

products gene name :

C3a

other gene names :

C3; C3; ASP; Plp; HSE-MSF; AI255234; ASP

uniprot entry name :

CO3_MOUSE

host :

E Coli

sequence length :

1663

sequence :

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-SVQLMERRMD KAGQYTDKGL RKCCEDGMRD IPMRYSCQRR ARLITQGENC IKAFIDCCNH ITKLREQHRR DHVLGLAR

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Ser671~Arg748 (Accession # P01027), with two N-terminal Tags, His-tag and GST-tag. Subcellular Location: Secreted. Predicted isoelectric point: 7.6. Predicted Molecular Mass: 41.2kDa

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Accurate Molecular Mass: 38kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in ddH2O.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

126518317

ncbi acc num :

NP_033908.2

ncbi gb acc num :

NM_009778.2

uniprot acc num :

P01027

ncbi mol weight :

41.2kDa

ncbi pathways :

Activation Of C3 And C5 Pathway (926093); Adaptive Immune System Pathway (926013); Alternative Complement Activation Pathway (926092); Chagas Disease (American Trypanosomiasis) Pathway (147810); Chagas Disease (American Trypanosomiasis) Pathway (147795); Class A/1 (Rhodopsin-like Receptors) Pathway (926991); Complement Activation, Classical Pathway (198379); Complement And Coagulation Cascades Pathway (198335); Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484)

ncbi summary :

This gene encodes complement protein C3 which plays a central role in the classical, alternative and lectin activation pathways of the complement system. The encoded preproprotein undergoes a multi-step processing to generate various functional peptides. Mice deficient in the encoded protein fail to clear bacteria from the blood stream upon infection, display diminished airway hyperresponsiveness and lung eosinophilia upon allergen-induced pulmonary allergy, and develop severe lung injury after deposition of IgG immune complexes. Deficiency of the homolog of the encoded protein in humans was found to be associated with increased susceptibility to infections, age-related macular degeneration, and atypical hemolytic uremic syndrome. [provided by RefSeq, Mar 2015]

uniprot summary :

C3: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Defects in C3 are the cause of complement component 3 deficiency (C3D). A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis. Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin- containing structure known as Bruch membrane. Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage. Protein type: Secreted, signal peptide; Inhibitor; Secreted. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding; endopeptidase inhibitor activity; cofactor binding; lipid binding; C5L2 anaphylatoxin chemotactic receptor binding. Biological Process: positive regulation of developmental growth; immune system process; complement activation, alternative pathway; fatty acid metabolic process; complement activation; positive regulation of angiogenesis; positive regulation of activation of membrane attack complex; positive regulation of G-protein coupled receptor protein signaling pathway; positive regulation of type IIa hypersensitivity; positive regulation of phagocytosis; innate immune response; positive regulation of protein amino acid phosphorylation; lipid metabolic process; blood coagulation; inflammatory response; complement activation, classical pathway

size1 :

0.01 mg

price1 :

185 USD

size2 :

0.05 mg

price2 :

390

size3 :

0.1 mg

price3 :

605

more info or order :

MyBioSource product webpage