Recombinant Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) | MyBioSource MBS2011705 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)

catalog :

MBS2011705

quantity :

0.01 mg

price :

200 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2011705

products type :

Recombinant Protein

products full name :

Recombinant Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)

products short name :

Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)

other names :

Metalloproteinase inhibitor 2; Metalloproteinase inhibitor 2; metalloproteinase inhibitor 2; tissue inhibitor of metalloproteinases 2; tissue inhibitor of metalloproteinase 2; Tissue inhibitor of metalloproteinases 2

products gene name :

TIMP2

other gene names :

Timp2; Timp2; Timp-2; D11Bwg1104e; Timp-2; TIMP-2

uniprot entry name :

TIMP2_MOUSE

host :

E Coli

sequence length :

220

sequence :

The sequence of the target protein is listed below. CSCS PVHPQQAFCN ADVVIRAKAV SEKEVDSGND IYGNPIKRIQ YEIKQIKMFK GPDKDIEFIY TAPSSAVCGV SLDVGGKKEY LIAGKAEGDG KMHITLCDFI VPWDTLSITQ KKSLNHRYQM GCECKITRCP MIPCYISSPD ECLWMDWVTE KSINGHQAKF FACIKRSDGS CAWYRGAAPP KQEFLDIEDP

purity :

> 95%

form :

Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Cys27~Pro220 (Accession # P25785) with N-terminal His-Tag. Predicted isoelectric point: 6.5

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

267133

ncbi acc num :

P25785.2

uniprot acc num :

P25785

ncbi mol weight :

23.2kDa

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (927075); Degradation Of The Extracellular Matrix Pathway (927074); Extracellular Matrix Organization Pathway (927061); Matrix Metalloproteinases Pathway (198370)

uniprot summary :

TIMP2: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Interacts (via the C-terminal) with MMP2 (via the C- terminal PEX domain); the interaction inhibits the MMP2 activity. Down-regulated by TGFB1. Belongs to the protease inhibitor I35 (TIMP) family. Protein type: Cell development/differentiation; Secreted; Extracellular matrix; Secreted, signal peptide; Motility/polarity/chemotaxis; Inhibitor. Cellular Component: extracellular space; neuron projection; cell surface; growth cone; cell soma; extracellular region; basement membrane. Molecular Function: metalloendopeptidase inhibitor activity; enzyme inhibitor activity; integrin binding; protease binding; protease inhibitor activity; metal ion binding; enzyme activator activity. Biological Process: regulation of Rap protein signal transduction; negative regulation of proteolysis; positive regulation of catalytic activity; negative regulation of metalloenzyme activity; regulation of neuron differentiation; regulation of cAMP metabolic process; response to hormone stimulus; negative regulation of peptidase activity; negative regulation of membrane protein ectodomain proteolysis; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; response to cytokine stimulus; regulation of MAPKKK cascade; negative regulation of Ras protein signal transduction; negative regulation of mitotic cell cycle; positive regulation of adenylate cyclase activity; positive regulation of neuron differentiation

size1 :

0.01 mg

price1 :

200 USD

size2 :

0.05 mg

price2 :

430

size3 :

0.1 mg

price3 :

665

more info or order :

MyBioSource product webpage