Recombinant Hypoxia Inducible Factor 2 Alpha (HIF2a) | MyBioSource MBS2011699 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Hypoxia Inducible Factor 2 Alpha (HIF2a)

catalog :

MBS2011699

quantity :

0.01 mg

price :

145 USD

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

product information

catalog number :

MBS2011699

products type :

Recombinant Protein

products full name :

Recombinant Hypoxia Inducible Factor 2 Alpha (HIF2a)

products short name :

[Hypoxia Inducible Factor 2 Alpha (HIF2a)]

other names :

[endothelial PAS domain-containing protein 1; Endothelial PAS domain-containing protein 1; endothelial PAS domain-containing protein 1; EPAS-1; HIF2 alpha; HIF2-alpha; HIF-2 alpha; HIF-2-alpha; hypoxia inducible factor 2a; hypoxia-inducible factor 2 alpha; hypoxia-inducible factor 2-alpha; hypoxia inducible factor 2, alpha subunit; endothelial PAS domain protein 1; Hypoxia-inducible factor 2-alpha]

products gene name :

[HIF2a]

other gene names :

[Epas1; Epas1; HLF; Hif2a; Hif2a; EPAS-1; HIF-2-alpha; HIF2-alpha]

uniprot entry name :

EPAS1_RAT

host :

E.coli

purity :

> 97%

form :

20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

concentration :

Original Concentration: 2000ug/mL

storage stability :

Storage:. Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test:. The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Positive Control; Immunogen; SDS-PAGE; Wesetn Blot (WB).

app notes :

(May be suitable for use in other assays to be determined by the end user.)

image1 heading :

Sequence

image2 heading :

SDS-PAGE

other info1 :

Organism Species: Rattus norvegicus (Rat). Source: Prokaryotic expression. Residues: Arg24~Glu348. Tags: N-terminal His Tag. Subcellular Location: Nucleus

other info2 :

Traits: Freeze-dried powder. Predicted isoelectric point: 6.5. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

12831205

ncbi acc num :

NP_075578.1

ncbi gb acc num :

NM_023090.1

uniprot acc num :

Q9JHS1

ncbi mol weight :

Predicted Molecular Mass: 38.5kDa. Accurate Molecular Mass: 38kDa as determined by SDS-PAGE reducing conditions.

ncbi pathways :

Adipogenesis Pathway (198479); Cellular Response To Hypoxia Pathway (935702); Cellular Responses To Stress Pathway (935701); Oxygen-dependent Asparagine Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway (935704); Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway (935705); Pathways In Cancer (83493); Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway (935706); Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway (935703); Renal Cell Carcinoma Pathway (83495); Renal Cell Carcinoma Pathway (519)

ncbi summary :

activates luciferase activity after transfection [RGD, Feb 2006]

uniprot summary :

Function: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD . By similarity. Subunit structure: Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT . By similarity. Subcellular location: Nucleus . Potential. Post-translational modification: In normoxia, is probably hydroxylated on Pro-405 and Pro-530 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization . By similarity.In normoxia, is hydroxylated on Asn-851 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation . By similarity.Phosphorylated on multiple sites in the CTAD . By similarity.The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains . By similarity. Sequence similarities: Contains 1 bHLH (basic helix-loop-helix) domain.Contains 1 PAC (PAS-associated C-terminal) domain.Contains 2 PAS (PER-ARNT-SIM) domains.

size1 :

0.01 mg

price1 :

145 USD

size2 :

0.05 mg

price2 :

275

size3 :

0.1 mg

price3 :

430

size4 :

0.2 mg

price4 :

525

size5 :

0.5 mg

price5 :

1015

size6 :

1 mg

price6 :

1515

more info or order :

MyBioSource product webpage