protein

Recombinant Collagen Type VI Alpha 1 (COL6a1)

MBS2011694

0.01 mg

185 USD

Recombinant Protein

Recombinant Collagen Type VI Alpha 1 (COL6a1)

Collagen Type VI Alpha 1 (COL6a1)

collagen alpha-1(VI) chain; Collagen alpha-1(VI) chain; collagen alpha-1(VI) chain; collagen alpha-1(VI) chain; procollagen, type VI, alpha 1; collagen, type VI, alpha 1

COL6a1

Col6a1; Col6a1; Col6a-1; AI747156

CO6A1_MOUSE

E Coli

1025

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-TCPIT FSSPADITIL LDSSASVGSH NFETTKVFAK RLAERFLSAG RADPSQDVRV AVVQYSGQGQ QQPGRAALQF LQNYTVLASS VDSMDFINDA TDVNDALSYV TRFYREASSG ATKKRVLLFS DGNSQGATAE AIEKAVQEAQ RAGIEIFVVV VGPQVNEPHI RVLVTGKTAE YDVAFGERHL FR

> 95%

Supplied as lyophilized form in PBS, pH7.4, containing 1 mM DTT, 5% trehalcse, 0.01 % sarcosyl and preservative.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Thr816~Arg1002 (Accession # Q04857) with N-terminal His-Tag. Subcellular Location: Secreted, extracellular space, extracellular matrix.

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Predicted isoelectric point: 6.1

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

6753484

NP_034063.1

NM_009933.4

Q04857

21.8kDa

Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (927064); Axon Guidance Pathway (926383); Collagen Biosynthesis And Modifying Enzymes Pathway (927063); Collagen Formation Pathway (927062); Developmental Biology Pathway (926382); ECM-receptor Interaction Pathway (83265); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (927061); Focal Adhesion Pathway (83264); Focal Adhesion Pathway (478)

COL6A1: Collagen VI acts as a cell-binding protein. Defects in COL6A1 are a cause of Bethlem myopathy (BM). BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles. Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD); also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy. Belongs to the type VI collagen family. Protein type: Extracellular matrix; Secreted, signal peptide; Secreted. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; protein complex; collagen; membrane; lysosomal membrane; extracellular region; sarcolemma. Molecular Function: platelet-derived growth factor binding. Biological Process: osteoblast differentiation; cell adhesion

0.01 mg

185 USD

0.05 mg

385

0.1 mg

595