Recombinant Glycogen Phosphorylase, Muscle (PYGM) | MyBioSource MBS2011599 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Glycogen Phosphorylase, Muscle (PYGM)

catalog :

MBS2011599

quantity :

0.01 mg

price :

150 USD

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

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product information

catalog number :

MBS2011599

products type :

Recombinant Protein

products full name :

Recombinant Glycogen Phosphorylase, Muscle (PYGM)

products short name :

[Glycogen Phosphorylase, Muscle (PYGM)]

other names :

[glycogen phosphorylase, muscle form; Glycogen phosphorylase, muscle form; glycogen phosphorylase, muscle form; myophosphorylase; muscle glycogen phosphorylase; Myophosphorylase]

products gene name :

[PYGM]

other gene names :

[Pygm; Pygm; PG; AI115133]

uniprot entry name :

PYGM_MOUSE

host :

E Coli

sequence length :

842

sequence :

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-RKQISVRGLA GVENVSELKK NFNRHLHFTL VKDRNVATPR DYYFALAHTV RDHLVGRWIR TQQHYYEKDP KRIYYLSLEF YMGRTLQNTM VNLALENACD EATYQLGLDM EELEEIEEDA GLGNGGLGRL AACFLDSMAT LGLAAYGYGI RYEFGIFNQK ICGGWQMEEA DDWLRYG

purity :

>98%

form :

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), CoIP, ReporterAssays, Purification, Amine Reactive Labeling.

image1 heading :

Sequence Information

image2 heading :

SDS-Page

image3 heading :

Testing Data

other info1 :

Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Arg11~Gly187 with N-terminal His-Tag. Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Predicted isoelectric point: 6.0. Predicted Molecular Mass: 21.7kDa. Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex. Tissue Specificity: Skeletal Muscle, Brain, Heart, Kidney. Traits: Freeze-dried powder

other info2 :

Note: The possible reasons that the actual band size differs from the predicted are as follows:. 1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affect the charge of the protein. 3. Post-translational modification:Phosphorylation, glycosylation, methylation etc. 4. Post-translation clevage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc.

ncbi gi num :

6755256

ncbi acc num :

NP_035354.1

ncbi gb acc num :

NM_011224.1

uniprot acc num :

Q9WUB3

ncbi mol weight :

Predicted Molecular Weight: 22.0kDa. Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions.

ncbi pathways :

Glucose Metabolism Pathway (926672); Glycogen Metabolism Pathway (198321); Glycogen Breakdown (glycogenolysis) Pathway (926676); Insulin Signaling Pathway (83287); Insulin Signaling Pathway (501); Metabolism Pathway (926669); Metabolism Of Carbohydrates Pathway (926670); Starch And Sucrose Metabolism Pathway (83173); Starch And Sucrose Metabolism Pathway (344)

uniprot summary :

PYGM: an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Dimers associate into a tetramer to form the enzymatically active phosphorylase A. Phosphorylation of Ser-14 converts phosphorylase B (unphosphorylated) to phosphorylase A. Protein type: Phosphorylase; Transferase; EC 2.4.1.1; Carbohydrate Metabolism - starch and sucrose; Endoplasmic reticulum. Cellular Component: sarcoplasmic reticulum; cytoplasm; Z disc. Molecular Function: transferase activity; glycogen phosphorylase activity; transferase activity, transferring glycosyl groups; phosphorylase activity; nucleotide binding; drug binding; carbohydrate binding; catalytic activity; pyridoxal phosphate binding; AMP binding. Biological Process: cellular calcium ion homeostasis; glycogen metabolic process; response to organic substance; glycogen catabolic process; response to cAMP; metabolic process; carbohydrate metabolic process; response to hypoxia

size1 :

0.01 mg

price1 :

150 USD

size2 :

0.05 mg

price2 :

295

size3 :

0.1 mg

price3 :

465

size4 :

0.2 mg

price4 :

580

size5 :

0.5 mg

price5 :

1120

size6 :

1 mg

price6 :

1665

more info or order :

MyBioSource product webpage