protein

Recombinant Patched 1 (PTCH1)

MBS2011579

0.01 mg

140 USD

Recombinant Protein

Recombinant Patched 1 (PTCH1)

[Patched 1 (PTCH1)]

[protein patched homolog 1; Protein patched homolog 1; protein patched homolog 1; Patched 1; Ptch1 protein +12b; mesenchymal dysplasia; patched homolog 1]

[PTCH1]

[Ptch1; Ptch1; Ptc; mes; Ptc1; Ptch; A230106A15Rik; Ptch; PTC; PTC1]

PTC1_MOUSE

E Coli

1434

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- LIITPLDC FWEGAKLQSG TAYLLGKPPL RWTNFDPLEF LEELKKINYQ VDSWEEMLNK AEVGHGYMDR PCLNPADPDC PATAPNKNST KPLDVALVLN GGCQGLSRKY MHWQEELIVG GTVKNATGKL VSAHALQTMF QLMTPKQMYE HFRGYDYVSH INWNEDRAAA ILEAWQRTYV EVVHQSVAPN STQKVLPFTT TTLDDILKSF SD

> 95%

Supplied as lyophilized form in 20mM Tris, 500mM NaCI, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

SDS-Page

Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Leu213~Asp422 (Accession # Q61115) with two N-terminal Tags, His-tag and T7-tag. Usage: Reconstitute in ddH2O

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Predicted Isoelectric Point: 6.3. Subcellular Location: Membrane; Multi-pass membrane protein.

6679519

NP_032983.1

NM_008957.2

Q61115

27.3kDa

Basal Cell Carcinoma Pathway (83306); Basal Cell Carcinoma Pathway (525); Class B/2 (Secretin Family Receptors) Pathway (927016); Endochondral Ossification Pathway (198336); GPCR Ligand Binding Pathway (926990); Hedgehog Signaling Pathway (198414); Hedgehog Signaling Pathway (83260); Hedgehog Signaling Pathway (474); Pathways In Cancer (83298); Proteoglycans In Cancer Pathway (782024)

PTCH1: a multi-pass membrane protein member of the ?patched? family that acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog protein?s signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. Interacts with SNX17. Expressed in tumor cells but not in normal skin. In the embryo, found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud. Defects in PTCH1 are the cause of basal cell nevus syndrome (BCNS), also known as Gorlin syndrome. BCNS is an autosomal dominant disease characterized by nevoid basal cell carcinomas and developmental abnormalities such as rib and craniofacial alterations, polydactyly, syndactyly, and spina bifida. In addition, the patients suffer from a multitude of tumors like basal cell carcinomas, fibromas of the ovaries and heart, cysts of the skin, jaws and mesentery, as well as medulloblastomas and meningiomas. PTCH1 defects is also the cause of holoprosencephaly, the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Protein type: Tumor suppressor; Membrane protein, multi-pass; Membrane protein, integral; Cell cycle regulation. Cellular Component: Golgi apparatus; membrane; intracellular membrane-bound organelle; integral to plasma membrane; perinuclear region of cytoplasm; postsynaptic density; extracellular region; integral to membrane; plasma membrane; caveola; midbody. Molecular Function: heparin binding; protein binding; cyclin binding; zinc ion binding; hedgehog receptor activity; cholesterol binding; protein complex binding; patched binding; smoothened binding. Biological Process: heart morphogenesis; hindlimb morphogenesis; epidermis development; regulation of mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; cell fate determination; glucose homeostasis; signal transduction; response to chlorate; negative regulation of cell proliferation; regulation of protein localization; mammary gland development; regulation of growth; negative regulation of osteoblast differentiation; dorsoventral neural tube patterning; negative regulation of epithelial cell proliferation; embryonic limb morphogenesis; smoothened signaling pathway; epidermal cell fate specification; negative regulation of multicellular organism growth; pharyngeal system development; in utero embryonic development; negative regulation of transcription factor activity; neural tube patterning; negative regulation of cell division; keratinocyte proliferation; spinal cord motor neuron differentiation; limb morphogenesis; pattern specification process; regulation of cell proliferation; organ morphogenesis; dorsal/ventral pattern formation; ureteric bud branching; neural plate pattern formation; negative regulation of smoothened signaling pathway; positive regulation of epidermal cell differentiation; neural tube closure; embryonic organ development; protein processing; brain development; neural tube formation; regulation of smoothened signaling pathway

0.01 mg

140 USD

0.05 mg

265

0.1 mg

405

0.2 mg

500

0.5 mg

970

1 mg

1435