catalog number :
MBS2011550
products type :
Recombinant Protein
products full name :
Recombinant Galectin 8 (GAL8)
products short name :
Galectin 8 (GAL8)
other names :
galectin-8 isoform a; Galectin-8; galectin-8; galectin 8; galectin-8g; Po66 carbohydrate binding protein; po66 carbohydrate-binding protein; prostate carcinoma tumor antigen 1; lectin, galactoside-binding, soluble, 8; Po66 carbohydrate-binding protein; Po66-CBP; Prostate carcinoma tumor antigen 1
products gene name :
GAL8
other gene names :
LGALS8; LGALS8; Gal-8; PCTA1; PCTA-1; Po66-CBP; Gal-8; Po66-CBP; PCTA-1
uniprot entry name :
LEG8_HUMAN
sequence :
The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEFELRRQ-MMLSLNNLQN IIYNPVIPFV GTIPDQLDPG TLIVIRGHVP SDADRFQVDL QNGSSMKPRA DVAFHFNPRF KRAGCIVCNT LINEKWGREE ITYDTPFKRE KSFEIVIMVL KDKFQVAVNG KHTLLYGHRI GPEKIDTLGI YGKVNIHSIG FSFSSDLQST QASSLELTEI SRENVPKSGT PQLRLPFAAR LNTPMGPGRT VVVKGEVNAN AKSFNVDLLA GKSKDIALHL NPRLNIKAFV RNSFLQESWG EEERNITSFP FSPGMYFEMI IYCDVREFKV AVNGVHSLEY KHRFKELSSI DTLEINGDIH LLEVRSW
form :
Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
storage stability :
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
other info1 :
Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Met1~Trp317 (Accession # O00214) with N-terminal His-Tag
other info2 :
Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Predicted isoelectric point: 8.4
products description :
About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.
ncbi acc num :
NP_006490.3
ncbi gb acc num :
NM_006499.4
ncbi mol weight :
38.0kDa
ncbi summary :
This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
uniprot summary :
LGALS8: 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion. Chromosomal Location of Human Ortholog: 1q43. Cellular Component: extracellular space; membrane; cytoplasm. Molecular Function: protein binding; carbohydrate binding. Biological Process: plasma cell differentiation; T cell costimulation