Recombinant A Disintegrin And Metalloprotease 17 (ADAM17) | MyBioSource MBS2011531 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant A Disintegrin And Metalloprotease 17 (ADAM17)

catalog :

MBS2011531

quantity :

0.01 mg

price :

185 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2011531

products type :

Recombinant Protein

products full name :

Recombinant A Disintegrin And Metalloprotease 17 (ADAM17)

products short name :

A Disintegrin And Metalloprotease 17 (ADAM17)

other names :

disintegrin and metalloproteinase domain-containing protein 17 preproprotein; Disintegrin and metalloproteinase domain-containing protein 17; disintegrin and metalloproteinase domain-containing protein 17; TNF-alpha convertase; snake venom-like protease; TNF-alpha converting enzyme; ADAM metallopeptidase domain 18; tumor necrosis factor, alpha, converting enzyme; ADAM metallopeptidase domain 17; Snake venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme

products gene name :

ADAM17

other gene names :

ADAM17; ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B; CSVP; TACE; ADAM 17

uniprot entry name :

ADA17_HUMAN

host :

E Coli

sequence length :

824

sequence :

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF-KLLVVAD HRFYRYMGRG EESTTTNYLI ELIDRVDDIY RNTSWDNAGF KGYGIQIEQI RILKSPQEVK PGEKHYNMAK SYPNEEKDAW DVKMLLEQFS FDIAEEASKV CLAHLFTYQD FDMGTLGLAY VGSPRANSHG GVCPKAYYSP VGKKNIYLNS GLTSTKNYGK TILTKEADLV TTHELGHNFG AEHDPDGLAE CAPNEDQGGK YVMYPIAVSG DHENNKMFSN CSKQSIYKTI ESKAQECFQE R

purity :

> 95%

form :

Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Lys226~Arg473 (Accession # P78536) with N-terminal His-Tag

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

73747889

ncbi acc num :

NP_003174.3

ncbi gb acc num :

NM_003183.4

uniprot acc num :

P78536

ncbi mol weight :

29.5kDa

ncbi pathways :

Activated NOTCH1 Transmits Signal To The Nucleus Pathway (576270); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Apoptosis Pathway (105648); BDNF Signaling Pathway (712093); Collagen Degradation Pathway (730309); Constitutive Signaling By NOTCH1 HD Domain Mutants Pathway (771591); Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway (771595); Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway (771593)

ncbi summary :

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq, Jul 2008]

uniprot summary :

TACE: a type I membrane protein with disintegrin and metalloprotease activity. An ubiquitously expressed ectoenzyme of peptidase family M12B. Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Possesses a narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala- -Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha) to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, and the amyloid precursor protein. Also involved in the activation of Notch pathway. Binds 1 zinc ion per subunit. Two splice variant isoforms have been described. Protein type: EC 3.4.24.86; Motility/polarity/chemotaxis; Protease; Membrane protein, integral. Chromosomal Location of Human Ortholog: 2p25. Cellular Component: focal adhesion; cell surface; membrane; integral to plasma membrane; cytoplasm; apical plasma membrane; plasma membrane; intercellular junction; lipid raft; actin cytoskeleton. Molecular Function: integrin binding; protein binding; interleukin-6 receptor binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; Notch binding; SH3 domain binding; PDZ domain binding. Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; neutrophil mediated immunity; T cell differentiation in the thymus; cell motility involved in cell locomotion; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; proteolysis; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; extracellular matrix disassembly; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; germinal center formation; negative regulation of interleukin-8 production; cell adhesion; response to drug; spleen development; epidermal growth factor receptor signaling pathway; Notch signaling pathway; response to high density lipoprotein stimulus; membrane protein intracellular domain proteolysis; membrane protein ectodomain proteolysis; positive regulation of transforming growth factor beta receptor signaling pathway; Notch receptor processing; positive regulation of cell motility; positive regulation of cell growth; positive regulation of chemokine production; regulation of mast cell apoptosis; collagen catabolic process; PMA-inducible membrane protein ectodomain proteolysis; B cell differentiation; response to hypoxia; cell adhesion mediated by integrin; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration; wound healing, spreading of epidermal cells. Disease: Inflammatory Skin And Bowel Disease, Neonatal, 1

size1 :

0.01 mg

price1 :

185 USD

size2 :

0.05 mg

price2 :

380

size3 :

0.1 mg

price3 :

585

more info or order :

MyBioSource product webpage