Recombinant Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2) | MyBioSource MBS2011514 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2)

catalog :

MBS2011514

quantity :

0.01 mg

price :

205 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2011514

products type :

Recombinant Protein

products full name :

Recombinant Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2)

products short name :

Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2)

other names :

ectonucleotide pyrophosphatase/phosphodiesterase family member 2 isoform 2 preproprotein; Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; autotaxin-t; plasma lysophospholipase D; extracellular lysophospholipase D; phosphodiesterase I/nucleotide pyrophosphatase 2; ectonucleotide pyrophosphatase/phosphodiesterase 2; Autotaxin; Extracellular lysophospholipase D

products gene name :

ENPP2

other gene names :

ENPP2; ENPP2; ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA; ATX; PDNP2; E-NPP 2; LysoPLD

uniprot entry name :

ENPP2_HUMAN

host :

E Coli

sequence length :

863

sequence :

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-RLHT KGSTEERHLL YGRPAVLYRT RYDILYHTDF ESGYSEIFLM PLWTSYTVSK QAEVSSVPDH LTSCVRPDVR VSPSFSQNCL AYKNDKQMSY GFLFPPYLSS SPEAKYDAFL VTNMVPMYPA FKRVWNYFQR VLVKKYASER NGVNVISGPI FDYDYDGLHD TEDKIKQYVE GSSIPVPTHY YSIITSCLDF TQPADKCDGP LSVSSFILPH RPDNEESCNS SEDESKWVEE LMKMHTARVR DIEHLTSLDF FRKTSRSYPE ILTLK

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). (May be suitable for use in other assays to be determined by the end user.)

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Arg637~Lys905 (Accession # Q13822) with two N-terminal Tags, His-tag and T7-tag. Predicted isoelectric point: 6.5

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. USAGE: Reconstitute in ddH2O.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

91823602

ncbi acc num :

NP_001035181.1

ncbi gb acc num :

NM_001040092.2

uniprot acc num :

Q13822

ncbi mol weight :

35.0kDa

ncbi pathways :

Ether Lipid Metabolism Pathway (82990); Ether Lipid Metabolism Pathway (365)

ncbi summary :

The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2008]

uniprot summary :

ENPP2: Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility- related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Phosphodiesterase; Lipid Metabolism - ether lipid; Secreted, signal peptide; EC 3.1.4.39; Secreted; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 8q24.1. Cellular Component: extracellular space; integral to plasma membrane; plasma membrane. Molecular Function: phosphodiesterase I activity; nucleotide diphosphatase activity; nucleic acid binding; zinc ion binding; hydrolase activity; calcium ion binding; transcription factor binding; scavenger receptor activity; alkylglycerophosphoethanolamine phosphodiesterase activity; polysaccharide binding; lysophospholipase activity. Biological Process: receptor-mediated endocytosis; G-protein coupled receptor protein signaling pathway; phospholipid catabolic process; immune response; chemotaxis; cell motility; regulation of cell migration; regulation of angiogenesis; phosphate metabolic process

size1 :

0.01 mg

price1 :

205 USD

size2 :

0.05 mg

price2 :

460

size3 :

0.1 mg

price3 :

715

more info or order :

MyBioSource product webpage