Recombinant Semaphorin 3F (SEMA3F) | MyBioSource MBS2011488 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Semaphorin 3F (SEMA3F)

catalog :

MBS2011488

quantity :

0.01 mg

price :

165 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2011488

products type :

Recombinant Protein

products full name :

Recombinant Semaphorin 3F (SEMA3F)

products short name :

Semaphorin 3F (SEMA3F)

other names :

semaphorin-3F; Semaphorin-3F; semaphorin-3F; sema IV; sema III/F; semaphorin IV; semaphorin III/F; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3F; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F; Sema III/F; Semaphorin IV

products gene name :

SEMA3F

other gene names :

SEMA3F; SEMA3F; SEMA4; SEMAK; SEMA-IV; Sema IV

uniprot entry name :

SEM3F_HUMAN

host :

E Coli

sequence length :

785

sequence :

The target protein is fused with N-terminal His-Tag, its sequence is listed below. V RNPVIYAVFT SSGSVFRGSA VCVYSMADIR MVFNGPFAHK EGPNYQWMPF SGKMPYPRPG TCPGGTFTPS MKSTKDYPDE VINFMRSHPL MYQAVYPLQR RPLVVRTGAP YRLTTIAVDQ VDAADGRYEV LFL

purity :

> 95%

form :

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), CoIP, Reporter Assays, Purification, Amine Reactive Labeling.

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Val350~Leu486. Predicted Molecular Mass: 16.6kDa. Accurate Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions.

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. Predicted Molecular Mass: 16.6kDa. Tags: N-terminal His-Tag. Homology: Mouse 96%, rat 96%. Tissue Specificity: Breast, kidney, brain, lung, liver. Subcellular Location: Secreted. Traits: Freeze-dried powder

ncbi gi num :

188497703

ncbi acc num :

NP_004177.3

ncbi gb acc num :

NM_004186.3

uniprot acc num :

Q13275

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476)

ncbi summary :

The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues (Kolodkin et al., 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al., 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al., 1995 [PubMed 7637782]), a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.[supplied by OMIM, Mar 2008]

uniprot summary :

SEMA3F: May play a role in cell motility and cell adhesion. Belongs to the semaphorin family. Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted. Chromosomal Location of Human Ortholog: 3p21.3. Cellular Component: extracellular space; membrane. Molecular Function: chemorepellent activity; receptor activity. Biological Process: axon extension involved in axon guidance; axon guidance; trigeminal nerve structural organization; facial nerve structural organization; branchiomotor neuron axon guidance; negative chemotaxis; nerve development; negative regulation of axon extension involved in axon guidance

size1 :

0.01 mg

price1 :

165 USD

size2 :

0.05 mg

price2 :

325

size3 :

0.1 mg

price3 :

505

more info or order :

MyBioSource product webpage