protein

Recombinant Transcription Factor A, Mitochondrial (TFAM)

MBS2011433

0.01 mg

165 USD

Recombinant Protein

Recombinant Transcription Factor A, Mitochondrial (TFAM)

[Transcription Factor A, Mitochondrial (TFAM)]

[transcription factor A, mitochondrial; Transcription factor A, mitochondrial; transcription factor A, mitochondrial; transcription factor A, mitochondrial]

[TFAM]

[Tfam; Tfam; Mttfa; mtTFA]

TFAM_RAT

E.coli

> 97%

Original Concentration 200 ug/mL

Storage: Avoid repeated freeze/thaw cycles. Store at 2.8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition

Positive Control; Immunogen; SDS.PAGE; WB. (May be suitable for use in other assays to be determined by the end user.

SDS-Page

Organism Species: Rattus norvegicus (Rat). Source: Prokaryotic expression. Residues: Ser43.Asn244. Tags: N.terminal His and GST Tag . Subcellular Location: Mitochondrion. Traits: Freeze.dried powder . Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

Predicted isoelectric point: 9.2. Predicted Molecular Mass: 53.7kDa. Accurate Molecular Mass: 53kDa as determined by SDS.PAGE reducing conditions. USAGE: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1.1.0 mg/mL. Do not vortex.

13786148

NP_112616.1

NM_031326.1

Q91ZW1

Gene Expression Pathway (936333); Huntington's Disease Pathway (83492); Huntington's Disease Pathway (512); Mitochondrial Gene Expression Pathway (219797); Mitochondrial Transcription Initiation Pathway (936354); RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway (936338); Transcription From Mitochondrial Promoters Pathway (936353)

regulates transcription and replication of mitochondrial DNA [RGD, Feb 2006]

Function: Isoform Mitochondrial binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA . By similarity. Isoform Nuclear may also function as a transcriptional activator or may have a structural role in the compaction of nuclear DNA during spermatogenesis . By similarity. Subunit structure: Monomer; binds DNA as a monomer. Interacts with TFB1M and TFB2M . By similarity. Subcellular location: Isoform Mitochondrial: Mitochondrion. Mitochondrion matrix mitochondrion nucleoid . By similarity. Isoform Nuclear: Nucleus. Tissue specificity: The mitochondrial isoform iswidely expressed while the nuclear isoform istestis-specific. Domain: Binds DNA via its HMG boxes. When bound to the mitochondrial light strand promoter, bends DNA into a U-turn shape, each HMG box bending the DNA by 90 degrees . By similarity. Post-translational modification: Phosphorylation by PKA within the HMG box 1 impairs DNA binding and promotes degradation by the AAA+ Lon protease . By similarity. Sequence similarities: Contains 2 HMG box DNA-binding domains.

0.01 mg

165 USD

0.05 mg

335

0.1 mg

515

0.2 mg

635

0.5 mg

1235

1 mg

1845