Recombinant Pyruvate Kinase, Muscle (PKM2) | MyBioSource MBS2011428 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Pyruvate Kinase, Muscle (PKM2)

catalog :

MBS2011428

quantity :

0.01 mg

price :

195 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2011428

products type :

Recombinant Protein

products full name :

Recombinant Pyruvate Kinase, Muscle (PKM2)

products short name :

Pyruvate Kinase, Muscle (PKM2)

other names :

pyruvate kinase PKM isoform c; Pyruvate kinase PKM; pyruvate kinase PKM; p58; OIP-3; tumor M2-PK; PK, muscle type; pyruvate kinase 2/3; OPA-interacting protein 3; pyruvate kinase isozymes M1/M2; pyruvate kinase muscle isozyme; thyroid hormone-binding protein 1; epididymis secretory protein Li 30; cytosolic thyroid hormone-binding protein; thyroid hormone-binding protein, cytosolic; pyruvate kinase, muscle; Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58

products gene name :

PKM2

other gene names :

PKM; PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30; OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1

uniprot entry name :

KPYM_HUMAN

host :

E Coli

reactivity :

OHomo sapiens (Human)

sequence length :

605

sequence :

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-KITLDNAYME KCDENILWLD YKNICKVVEV GSKIYVDDGL ISLQVKQKGA DFLVTEVENG GSLGSKKGVN LPGAAVDLPA VSEKDIQDLK FGVEQDVDMV FASFIRKA

purity :

> 95%

form :

Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Expression System: Prokaryotic expression. Residues: Lys141~Ala248 (Accession # P14618) with N-terminal His-Tag. Predicted isoelectric point: 5.4

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

ncbi gi num :

332164775

ncbi acc num :

NP_001193725.1

ncbi gb acc num :

NM_001206796.1

uniprot acc num :

P14618

ncbi mol weight :

13.4kDa

ncbi pathways :

Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (618580); Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (468242); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Glucose Metabolism Pathway (106199); Glycolysis Pathway (105911); Glycolysis (Embden-Meyerhof Pathway), Glucose = Pyruvate (413340); Glycolysis (Embden-Meyerhof Pathway), Glucose = Pyruvate (468194)

ncbi summary :

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]

uniprot summary :

PKM2: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. Overexpressed in a variety of cancers. Interacts with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. Binds Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogens is. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms of PKM2 have been reported. Protein type: EC 2.7.1.40; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; Nucleotide Metabolism - purine; Carbohydrate Metabolism - pyruvate; Mitochondrial. Chromosomal Location of Human Ortholog: 15q22. Cellular Component: extracellular matrix; mitochondrion; cytoplasm; plasma membrane; nucleus; cytosol; vesicle; cilium. Molecular Function: potassium ion binding; protein binding; magnesium ion binding; pyruvate kinase activity; ATP binding. Biological Process: glycolysis; carbohydrate metabolic process; programmed cell death; glucose metabolic process; pathogenesis; phosphorylation

size1 :

0.01 mg

price1 :

195 USD

size2 :

0.05 mg

price2 :

410

size3 :

0.1 mg

price3 :

635

more info or order :

MyBioSource product webpage