protein

Recombinant Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)

MBS2011413

0.01 mg

200 USD

Recombinant Protein

Recombinant Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)

Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)

protein Wnt-5a isoform 2; Protein Wnt-5a; protein Wnt-5a; wingless-related MMTV integration site 5A

WNT5A

Wnt5a; Wnt5a; Wnt-5a; 8030457G12Rik; Wnt-5a

WNT5A_MOUSE

E Coli

360

The sequence of the target protein is listed below. IIGAQPLCS QLAGLSQGQK KLCHLYQDHM QYIGEGAKTG IKECQYQFRH RRWNCSTVDN TSVFGRVMQI GSRETAFTYA VSAAGVVNAM SRACREGELS TCGCSRAARP KDLPRDWLWG GCGDNIDYGY RFAKEFVDAR ERERIHAKGS YESARILMNL HNNEAGRRTV YNLADVACKC HGVSGSCSLK TCWLQLADFR KVGDALKEKY DSAAAMRLNS RGKLVQVNSR FNSPTTQDLV YIDPSPDYCV RNESTGSLGT QGRLCNKTSE GMDGCELMCC GRGYDQFKTV QTERCHCKFH WCCYVKCKKC TEIVDQFVCK

> 95%

Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Ile62~Lys381 (Accession # P22725) with N-terminal GST-tag

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile ddH2O. Predicted isoelectric point: 8.3. Subcellular Location: Secreted, extracellular space, extracellular matrix.

371940979

NP_001243153.1

NM_001256224.1

P22725

62.2kDa

Basal Cell Carcinoma Pathway (83306); Basal Cell Carcinoma Pathway (525); Ca2+ Pathway (927049); Class B/2 (Secretin Family Receptors) Pathway (927016); ESC Pluripotency Pathways (198374); GPCR Ligand Binding Pathway (926990); HTLV-I Infection Pathway (373903); HTLV-I Infection Pathway (373889); Hedgehog Signaling Pathway (83260); Hedgehog Signaling Pathway (474)

WNT5A: Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes. Interacts with PORCN. Interacts with WLS. Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; cytoplasm; extracellular region; intracellular. Molecular Function: protein domain specific binding; protein binding; receptor tyrosine kinase-like orphan receptor binding; frizzled binding; cytokine activity; transcription factor activity; receptor binding; frizzled-2 binding. Biological Process: neural tube development; positive regulation of JNK activity; activation of MAPK activity; embryonic skeletal development; multicellular organismal development; positive regulation of transcription, DNA-dependent; negative chemotaxis; positive regulation of endocytosis; Wnt receptor signaling pathway through beta-catenin; negative regulation of synaptogenesis; positive regulation of interleukin-1 beta secretion; uterus development; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; negative regulation of BMP signaling pathway; anterior/posterior pattern formation; positive regulation of fibroblast proliferation; regulation of protein localization; cell-cell signaling; determination of anterior/posterior axis, embryo; convergent extension; positive regulation of mesenchymal cell proliferation; JNK cascade; embryonic limb morphogenesis; positive regulation of macrophage activation; positive regulation of cytokine production; inner ear morphogenesis; somitogenesis; cell fate commitment; urinary bladder development; olfactory bulb interneuron development; activation of protein kinase B; vagina development; positive regulation of interleukin-6 production; digestive tract morphogenesis; negative regulation of fat cell differentiation; organ morphogenesis; positive regulation of angiogenesis; genitalia development; Wnt receptor signaling pathway, calcium modulating pathway; positive regulation of protein catabolic process; midgut development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; embryonic digit morphogenesis; determination of left/right symmetry; negative regulation of transcription, DNA-dependent; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; axon guidance; wound healing; negative regulation of fibroblast growth factor receptor signaling pathway; hypophysis morphogenesis; positive regulation of cytokine secretion during immune response; positive regulation of JNK cascade; palate development; signal transduction; negative regulation of axon extension involved in axon guidance; activation of JNK activity; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; morphogenesis of an epithelium; establishment of planar polarity; heart looping; cell differentiation; negative regulation of epithelial cell proliferation; cervix development; cell migration; Wnt receptor signaling pathway; positive regulation of meiosis; Wnt receptor signaling pathway, planar cell polarity pathway; male gonad development; positive regulation of peptidyl-serine phosphorylation; limb morphogenesis; positive regulation of cGMP metabolic process; positive regulation of ossification; development of primary male sexual characteristics; convergent extension involved in organogenesis; embryonic development; positive regulation of interferon-gamma production; positive regulation of chemokine biosynthetic process; cartilage development; ameboidal cell migration; neural tube closure; hindgut morphogenesis; positive regulation of protein amino acid phosphorylation; neurite morphogenesis; positive regulation of inflammatory response; lung development

0.01 mg

200 USD

0.05 mg

440

0.1 mg

690