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Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12)

MBS2011347

0.01 mg

285 USD

MBS2011347

Recombinant Protein

Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12)

A Disintegrin And Metalloproteinase With Thrombospondin 12 (ADAMTS12)

A disintegrin and metalloproteinase with thrombospondin motifs 12; A disintegrin and metalloproteinase with thrombospondin motifs 12; A disintegrin and metalloproteinase with thrombospondin motifs 12; ADAM-TS12; ADAM-TS 12; metalloprotease disintegrin 12; a disintegrin-like and metalloprotease with thrombospondin type 1 motif, 12; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 12; a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 12; N/A

ADAMTS12

Adamts12; Adamts12; AI605170; ADAMTS-12; ADAM-TS 12; ADAM-TS12; ADAMTS-12

ATS12_MOUSE

E Coli

1600

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGS-SPA YWIVGNWSKC STTCGLGAYW RSVECSSGVD ADCTTIQRPD PAKKCHLRPC AGWRVGNWSK CSRNCSGGFK IREVQCMDSL DHHRSLRPFH CQFLAGAPPP LSMSCNLEPC GEWQVEPWSQ CSRSCGGGVQ ERGVSCPGGL CDWTKRPATT VPCNRHLCCH WATGNWELCN TSCGGGSQKR TIHCIPSENS TTEDQDQCLC DHQVKPPEFQ TCNQQACR

> 95%

Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Ser1318-Arg1538 (Accession # Q811B3) with N-terminal His-Tag

Endotoxin Level: Reconstitute in sterile PBS, pH7.2-pH7.4.

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

153945812

NP_780710.2

NM_175501.2

Q811B3

25.7kDa

Function: Metalloprotease that plays a role in the degradation of COMP . By similarity. Cleaves also alpha-2 macroglobulin and aggregan. Has anti-tumorigenic properties . By similarity. Cofactor: Binds 1 zinc ion per subunit . By similarity. Enzyme regulation: Inhibited by alpha-2 macroglobulin . By similarity. Subunit structure: Interacts with COMP . By similarity. Subcellular location: Secreted extracellular space extracellular matrix . By similarity. Domain: The C-terminal four TSP1-like repeats are necessary and sufficient for binding COMP . By similarity.The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix . By similarity.The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. Post-translational modification: The precursor is cleaved by a furin endopeptidase . By similarity.Subjected to an intracellular maturation process yielding a 120 kDa N-terminal fragment containing the metalloproteinase, disintegrin, one TSP type-1 and the Cys-rich domains and a 83 kDa C-terminal fragment containing the spacer 2 and four TSP type-1 domains . By similarity.Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion . By similarity. Sequence similarities: Contains 1 disintegrin domain.Contains 1 peptidase M12B domain.Contains 1 PLAC domain.Contains 8 TSP type-1 domains.

0.01 mg

285 USD