Recombinant C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF) | MyBioSource MBS2011343 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF)

catalog :

MBS2011343

quantity :

0.01 mg

price :

135 USD

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

SDS-PAGE

product information

catalog number :

MBS2011343

products type :

Recombinant Protein

products full name :

Recombinant C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF)

products short name :

[C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF)]

other names :

[RAF proto-oncogene serine/threonine-protein kinase; RAF proto-oncogene serine/threonine-protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Oncogene RAF1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; v-raf-1 murine leukemia viral oncogene homolog 1; Proto-oncogene c-RAF; cRaf; Raf-1]

products gene name :

[CRAF]

other gene names :

[RAF1; RAF1; NS5; CRAF; Raf-1; c-Raf; RAF; cRaf]

uniprot entry name :

RAF1_HUMAN

host :

E.coli

purity :

> 95%

form :

20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

concentration :

Original Concentration: 100ug/mL

storage stability :

Storage:. Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test:. The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Positive Control; Immunogen; SDS-PAGE; Western Blot (WB)

app notes :

(May be suitable for use in other assays to be determined by the end user.)

image1 heading :

Sequence

image2 heading :

SDS-PAGE

other info1 :

Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Trp342~Ser619. Tags: N-terminal His Tag. Subcellular Location: Membrane, Cytoplasm. Traits: Freeze-dried powder

other info2 :

Predicted isoelectric point: 9.2. Usage: Reconstitute in ddH2O to a concentration of 0.05-0.4 mg/mL. Do not vortex.

ncbi gi num :

4506401

ncbi acc num :

NP_002871.1

ncbi gb acc num :

NM_002880.3

uniprot acc num :

P04049

ncbi mol weight :

Predicted Molecular Mass: 35.5kDa. Accurate Molecular Mass: 35kDa as determined by SDS-PAGE reducing conditions.

ncbi pathways :

AGE/RAGE Pathway (698754); ARMS-mediated Activation Pathway (106466); Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (366160); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Axon Guidance Pathway (105688); B Cell Receptor Signaling Pathway (198909)

ncbi summary :

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]

uniprot summary :

RAF1: a proto-oncogenic TKL kinase of the RAF family. Functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate and activate MEK1/2, which in turn phosphorylate and activate ERK1/2. Acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MEK1/2) and the extracellular signal-regulated kinases (ERK1/2). RAF1, subsequent to phosphorylation by PAK1, phosphorylates BAD (Bcl2-antagonist of cell death) at S75. Phosphorylates adenylyl cyclases ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2 (cardiac muscle troponin T). Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (ASK1 and MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation. Protein type: Protein kinase, TKL; EC 2.7.11.1; Kinase, protein; Oncoprotein; Protein kinase, Ser/Thr (non-receptor); TKL group; RAF family. Chromosomal Location of Human Ortholog: 3p25. Cellular Component: Golgi apparatus; mitochondrial outer membrane; cytoplasm; plasma membrane; pseudopodium; cytosol; nucleus. Molecular Function: small GTPase binding; protein serine/threonine kinase activity; identical protein binding; protein binding; MAP kinase kinase kinase activity; mitogen-activated protein kinase kinase binding; protein heterodimerization activity; metal ion binding; ATP binding; protein kinase activity. Biological Process: axon guidance; nerve growth factor receptor signaling pathway; wound healing; activation of MAPKK activity; somatic stem cell maintenance; apoptosis; heart development; intermediate filament cytoskeleton organization and biogenesis; negative regulation of caspase activity; signal transduction; protein amino acid phosphorylation; regulation of apoptosis; negative regulation of cell proliferation; synaptic transmission; regulation of cell differentiation; small GTPase mediated signal transduction; transmembrane transport; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; adenylate cyclase activation; MAPKKK cascade; positive regulation of peptidyl-serine phosphorylation; cell proliferation; Ras protein signal transduction; response to hypoxia; insulin receptor signaling pathway; regulation of Rho protein signal transduction; innate immune response; blood coagulation; vascular endothelial growth factor receptor signaling pathway; negative regulation of protein complex assembly; negative regulation of apoptosis. Disease: Cardiomyopathy, Dilated, 1nn; Noonan Syndrome 5; Leopard Syndrome 2

size1 :

0.01 mg

price1 :

135 USD

size2 :

0.05 mg

price2 :

245

size3 :

0.1 mg

price3 :

365

size4 :

0.2 mg

price4 :

450

size5 :

0.5 mg

price5 :

870

size6 :

1 mg

price6 :

1280

more info or order :

MyBioSource product webpage