protein

Recombinant Angiopoietin 1 (ANGPT1)

MBS2011334

0.01 mg

150 USD

Recombinant Protein

Recombinant Angiopoietin 1 (ANGPT1)

[Angiopoietin 1 (ANGPT1)]

[angiopoietin-1; Angiopoietin-1; angiopoietin-1; angiopoietin 1]

[ANGPT1]

[Angpt1; Angpt1; Agpt; Agpt1; Ang-1; ANG-1]

ANGP1_RAT

E Coli

497

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-NNMPEPK KVFCNMDVNE GGWTVIQHRE DGSLDFQRGW KEYKMGFGNP SGEYWLGNEF IFAITSQRQY MLRIELMDWE GNRAYSQYDR FHIGNQKQNY RLYLKGHTGT AGKQSSLILH GADFSTKDAD NDNCMCKCAL MLTGGWWFDA

> 95%

PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE, WB, ELISA, IP, CoIP, ReporterAssays, Purification, Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

Sequence Information

SDS-Page

Organism: Rattus norvegicus (Rat). Expression System: Prokaryotic expression. Residues: Asn304~Ala450 with N-terminal His-Tag. Tissue Specificity: Placenta. Subcellular Location: Secreted. Traits: Freeze-dried powder

Predicted isoelectric point: 6.4. Predicted Molecular Mass: 18.3kDa. Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions. Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

23308739

NP_445998.1

NM_053546.1

O35460

Cell Surface Interactions At The Vascular Wall Pathway (935750); HIF-1 Signaling Pathway (695233); Hemostasis Pathway (935717); PI3K-Akt Signaling Pathway (692247); PI3K-Akt Signaling Pathway (692979); Rap1 Signaling Pathway (869320); Rap1 Signaling Pathway (878042); Ras Signaling Pathway (869318); Rheumatoid Arthritis Pathway (200306); Rheumatoid Arthritis Pathway (200269)

modulates endothelial cell survival and involved in the activation of endothelial cell-specific Tie-2 receptors [RGD, Feb 2006]

ANGPT1: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; microvillus; plasma membrane; lipid raft. Molecular Function: receptor tyrosine kinase binding; receptor binding. Biological Process: positive regulation of cell adhesion; transmembrane receptor protein tyrosine kinase activation (dimerization); positive regulation of receptor internalization; negative regulation of protein import into nucleus; negative regulation of cell adhesion; positive chemotaxis; ovarian follicle development; negative regulation of protein amino acid phosphorylation; response to vitamin B3; hemopoiesis; angiogenesis; negative regulation of neuron apoptosis; regulation of I-kappaB kinase/NF-kappaB cascade; cell differentiation; protein homooligomerization; regulation of endothelial cell proliferation; Tie receptor signaling pathway; organ regeneration; in utero embryonic development; positive regulation of blood vessel endothelial cell migration; positive regulation of peptidyl-serine phosphorylation; cell-substrate adhesion; negative regulation of cytokine secretion during immune response; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; heparin biosynthetic process; response to estrogen stimulus; positive regulation of protein ubiquitination; regulation of protein binding; response to hypoxia; negative regulation of vascular permeability; regulation of tumor necrosis factor production; regulation of satellite cell proliferation; sprouting angiogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

0.01 mg

150 USD

0.05 mg

295

0.1 mg

460

0.2 mg

570

0.5 mg

1105

1 mg

1645