Recombinant Caspase 1 (CASP1) | MyBioSource MBS2011263 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Caspase 1 (CASP1)

catalog :

MBS2011263

quantity :

0.01 mg

price :

170 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2011263

products type :

Recombinant Protein

products full name :

Recombinant Caspase 1 (CASP1)

products short name :

Caspase 1 (CASP1)

other names :

caspase-1; Caspase-1; caspase-1; p45; CASP-1; IL-1BC; IL-1B converting enzyme; IL-1 beta-converting enzyme; interleukin-1 beta convertase; interleukin 1 beta-converting enzyme; interleukin-1 beta-converting enzyme; caspase 1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45

products gene name :

CASP1

other gene names :

Casp1; Casp1; ICE; Il1bc; Il1bc; CASP-1; IL-1BC; ICE

uniprot entry name :

CASP1_MOUSE

host :

E Coli

reactivity :

Mus musculus (Mouse)

sequence length :

402

sequence :

The sequence of the target protein is listed below. NK EDGTFPGLTG TLKFCPLEKA QKLWKENPSE IYPIMNTTTR TRLALIICNT EFQHLSPRVG AQVDLREMKL LLEDLGYTVK VKENLTALEM VKEVKEFAAC PEHKTSDSTF LVFMSHGIQE GICGTTYSNE VSDILKVDTI FQMMNTLKCP SLKDKPKVII IQACRGEKQG VVLLKD

purity :

> 95%

form :

Supplied as lyophilized form in 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Residues: Asn119~Asp296. Expression System: Prokaryotic expression. Subcellular Location: Cytoplasm.: Accession # : P29452 with N-terminal His-Tag. Predicted isoelectric point: 6.8

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile ddH2O.

ncbi gi num :

86198305

ncbi acc num :

NP_033937.2

ncbi gb acc num :

NM_009807.2

uniprot acc num :

P29452

ncbi mol weight :

21.6kDa

ncbi pathways :

Amyotrophic Lateral Sclerosis (ALS) Pathway (83296); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Androgen Receptor Signaling Pathway (198319); Apoptosis Pathway (198339); Cytokine Signaling In Immune System Pathway (926145); Cytosolic DNA-sensing Pathway (125146); Cytosolic DNA-sensing Pathway (124833); IL-1 Signaling Pathway (198360); Immune System Pathway (926012); Inflammasomes Pathway (926098)

uniprot summary :

CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18. Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. Specifically inhibited by the cowpox virus Crma protein. Belongs to the peptidase C14A family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Apoptosis; EC 3.4.22.36; Protease. Cellular Component: neuron projection; protein complex; mitochondrion; cytoplasm; extracellular region; nucleus. Molecular Function: peptidase activity; protein binding; hydrolase activity; endopeptidase activity; cysteine-type peptidase activity. Biological Process: response to drug; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of apoptosis; apoptosis; membrane hyperpolarization; interleukin-1 beta production; microglial cell activation; response to lipopolysaccharide; positive regulation of interleukin-1 beta secretion; mitochondrial depolarization; proteolysis; response to organic cyclic substance; response to ATP; memory; regulation of apoptosis; positive regulation of interleukin-1 alpha secretion; response to bacterium; regulation of inflammatory response; response to hypoxia; regulation of autophagy; protein processing; positive regulation of circadian sleep/wake cycle, non-REM sleep; myoblast fusion; positive regulation of cytokine secretion

size1 :

0.01 mg

price1 :

170 USD

size2 :

0.05 mg

price2 :

345

size3 :

0.1 mg

price3 :

535

more info or order :

MyBioSource product webpage