Recombinant Phospholipase A2, Cytosolic (cPLA2) | MyBioSource MBS2011220 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Phospholipase A2, Cytosolic (cPLA2)

catalog :

MBS2011220

quantity :

0.01 mg

price :

190 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2011220

products type :

Recombinant Protein

products full name :

Recombinant Phospholipase A2, Cytosolic (cPLA2)

products short name :

Phospholipase A2, Cytosolic (cPLA2)

other names :

cytosolic phospholipase A2; Cytosolic phospholipase A2; cytosolic phospholipase A2; cPLA2; lysophospholipase; phospholipase A2 group IVA; phosphatidylcholine 2-acylhydrolase; calcium-dependent phospholipid-binding protein; phospholipase A2, group IVA (cytosolic, calcium-dependent); Phospholipase A2 group IVAIncluding the following 2 domains:Phospholipase A2 (EC:3.1.1.4)Alternative name(s):Phosphatidylcholine 2-acylhydrolase

products gene name :

cPLA2

other gene names :

PLA2G4A; PLA2G4A; PLA2G4; cPLA2-alpha; CPLA2; PLA2G4; cPLA2

uniprot entry name :

PA24A_HUMAN

host :

E Coli

sequence length :

749

sequence :

MSFIDPYQHI IVEHQYSHKF TVVVLRATKV TKGAFGDMLD TPDPYVELFI STTPDSRKRT RHFNNDINPV WNETFEFILD PNQENVLEIT LMDANYVMDE TLGTATFTVS SMKVGEKKEV PFIFNQVTEM VLEMSLEVCS CPDLRFSMAL CDQEKTFRQQ RKEHIRESMK KLLGPKNS

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). (May be suitable for use in other assays to be determined by the end user.)

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Met1~Ser178 (Accession # P47712) with N-terminal His-Tag. Subcellular Location: Cytoplasm. Cytoplasmic vesicle. Predicted isoelectric point: 5.9. Predicted Molecular Mass: 22.2kDa. Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions.

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Usage: Reconstitute in ddH2O.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

23943920

ncbi acc num :

NP_077734.1

ncbi gb acc num :

NM_024420.2

uniprot acc num :

P47712

ncbi mol weight :

22.2kDa

ncbi pathways :

ADP Signalling Through P2Y Purinoceptor 1 Pathway (161061); AGE/RAGE Pathway (698754); Acyl Chain Remodeling Of CL Pathway (645330); Acyl Chain Remodelling Of PC Pathway (645321); Acyl Chain Remodelling Of PE Pathway (645317); Acyl Chain Remodelling Of PG Pathway (645328); Acyl Chain Remodelling Of PI Pathway (645326); Acyl Chain Remodelling Of PS Pathway (645324); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553)

ncbi summary :

This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

uniprot summary :

cPLA2: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Arachidonic acid is a precursor for the eicosanoids that are involved in hemodynamic regulation, inflammatory responses, and other cellular processes. Promotes cerebellar long-term depression and motor learning. Translocates to the Golgi and endoplasmic reticulum in a calcium-dependent fashion. Translocation and activation of at the ER facilitates the process of ER stress. Regulates the biogenesis of lipid droplets, a process dependent on JNK and ceramide kinase. Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+. The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Inhibited when in trimolecular complex with ANXA2 and S100A10. Phosphorylation of S727 relieves this inhibitory interaction, thus activating PLA2G4A. Protein type: EC 3.1.1.5; Lipid Metabolism - glycerophospholipid; EC 3.1.1.4; Lipid Metabolism - linoleic acid; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - arachidonic acid; Phospholipase. Chromosomal Location of Human Ortholog: 1q25. Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; cytoplasm; mitochondrial inner membrane; cytoplasmic membrane-bound vesicle; cytosol. Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; calcium-dependent phospholipid binding; calcium ion binding; lysophospholipase activity. Biological Process: icosanoid biosynthetic process; platelet activation; platelet activating factor biosynthetic process; phospholipid catabolic process; phospholipid metabolic process; icosanoid metabolic process; glycerophospholipid biosynthetic process; arachidonic acid metabolic process; phosphatidic acid biosynthetic process; arachidonic acid secretion; blood coagulation; regulation of cell proliferation

size1 :

0.01 mg

price1 :

190 USD

size2 :

0.05 mg

price2 :

405

size3 :

0.1 mg

price3 :

630

more info or order :

MyBioSource product webpage