Recombinant Troponin T Type 3, Fast Skeletal (TNNT3) | MyBioSource MBS2011217 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Troponin T Type 3, Fast Skeletal (TNNT3)

catalog :

MBS2011217

quantity :

0.01 mg

price :

150 USD

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

product information

catalog number :

MBS2011217

products type :

Recombinant Protein

products full name :

Recombinant Troponin T Type 3, Fast Skeletal (TNNT3)

products short name :

[Troponin T Type 3, Fast Skeletal (TNNT3)]

other names :

[troponin T, fast skeletal muscle isoform 3; Troponin T, fast skeletal muscle; troponin T, fast skeletal muscle; fTnT; beta TnTF; beta-TnTF; troponin-T3, skeletal, fast; fast skeletal muscle troponin T; troponin T type 3 (skeletal, fast); Beta-TnTF; Fast skeletal muscle troponin T]

products gene name :

[TNNT3]

other gene names :

[TNNT3; TNNT3; TNTF; TnTf; fTnT]

uniprot entry name :

TNNT3_HUMAN

host :

E. Coli

reactivity :

Human

purity :

>98%

form :

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

concentration :

200ug/mL

storage stability :

Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, WB, ELISA, IP, CoIP, Purification, Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

image1 heading :

Sequence Information

image2 heading :

SDS-Page

other info1 :

Source: Prokaryotic expression. Residues: Arg147~Lys269. Tags: N-terminal His-Tag. Tissue Specificity: Skeletal Muscle, Liver, Lung. Traits: Freeze-dried powder.

other info2 :

Predicted isoelectric point: 10.1. Predicted Molecular Mass: 15.6kDa. Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

112789536

ncbi acc num :

NP_001036245.1

ncbi gb acc num :

NM_001042780.2

uniprot acc num :

P45378

ncbi mol weight :

18kDa

ncbi pathways :

Muscle Contraction Pathway (106261); Striated Muscle Contraction Pathway (198903); Striated Muscle Contraction Pathway (106262)

ncbi summary :

The binding of Ca(2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca(2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction. The troponin complex has protein subunits C, I, and T. Subunit C binds Ca(2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B). [provided by RefSeq, Oct 2009]

uniprot summary :

TNNT3: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNT3 are a cause of distal arthrogryposis type 2B (DA2B); also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. Belongs to the troponin T family. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Motor. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: troponin complex; cytosol. Molecular Function: troponin C binding; troponin I binding; tropomyosin binding; actin binding; calcium-dependent protein binding; calcium-dependent ATPase activity. Biological Process: skeletal muscle contraction; regulation of ATPase activity; metabolic process; regulation of striated muscle contraction; muscle filament sliding. Disease: Arthrogryposis, Distal, Type 2b

size1 :

0.01 mg

price1 :

150 USD

size2 :

0.05 mg

price2 :

290

size3 :

0.1 mg

price3 :

460

size4 :

0.2 mg

price4 :

565

size5 :

0.5 mg

price5 :

1095

size6 :

1 mg

price6 :

1630

more info or order :

MyBioSource product webpage