Recombinant Carbohydrate Antigen 19-9 (CA19-9) | MyBioSource MBS2011087 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Carbohydrate Antigen 19-9 (CA19-9)

catalog :

MBS2011087

quantity :

0.01 mg

price :

225 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2011087

products type :

Recombinant Protein

products full name :

Recombinant Carbohydrate Antigen 19-9 (CA19-9)

products short name :

Carbohydrate Antigen 19-9 (CA19-9)

other names :

galactoside 3(4)-L-fucosyltransferase; Galactoside 3(4)-L-fucosyltransferase; galactoside 3(4)-L-fucosyltransferase; galactoside 3(4)-L-fucosyltransferase; Lewis FT; fucosyltransferase III; alpha-(1,3/1,4)-fucosyltransferase; blood group Lewis alpha-4-fucosyltransferase; fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group); Blood group Lewis alpha-4-fucosyltransferase; Lewis FT; Fucosyltransferase 3; Fucosyltransferase III

products gene name :

CA19-9

other gene names :

FUT3; FUT3; LE; Les; FT3B; CD174; FucT-III; FT3B; LE; Lewis FT; FucT-III

uniprot entry name :

FUT3_HUMAN

host :

E Coli

sequence length :

361

sequence :

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-RVSRDD ATGSPRAPSG SSRQDTTPTR PTLLILLWTW PFHIPVALSR CSEMVPGTAD CHITADRKVY PQADTVIVHH WDIMSNPKSR LPPSPRPQGQ RWIWFNLEPP PNCQHLEALD RYFNLTMSYR SDSDIFTPYG WLEPWSGQPA HPPLNLSAKT ELVAWAVSNW KPDSARVRYY QSLQAHLKVD VYGRSHKPLP KGTMMETLSR YKFYLAFENS LHPDYITEKL WRNALEAWAV PVVLGPSRSN YERFLPPDAF IHVDDFQSPK DLARYLQELD KDHARYLSYF RWRETLRPRS FSWALDFCKA CWKLQQESRY QTVRSIAAWF T

purity :

> 95%

form :

Supplied as lyophilized form in PBS, pH7.4, containing 0.01% sarcosyl

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Arg35~Thr361 (Accession # P21217) with N-terminal His-Tag. Homology: Mouse 45%, rat 44%. Tissue Specificity: Stomach, intestine, lung, kidney. Subcellular Location: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Predicted isoelectric point: 9.0. Predicted Molecular Mass: 39.7kDa. Accurate Molecular Mass: 43kDa as determined by SDS-PAGE reducing conditions.

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Accurate Molecular Mass: 43kDa as determined by SDS-PAGE reducing conditions.

ncbi gi num :

4503809

ncbi acc num :

NP_000140.1

ncbi gb acc num :

NM_000149.3

uniprot acc num :

P21217

ncbi pathways :

Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway (82995); Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway (370)

ncbi summary :

The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

FUT3: May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links. Belongs to the glycosyltransferase 10 family. Protein type: EC 2.4.1.65; Transferase; Membrane protein, integral; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: membrane; integral to membrane. Molecular Function: 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase activity; alpha(1,3)-fucosyltransferase activity; fucosyltransferase activity. Biological Process: cell-cell recognition; biopolymer glycosylation; oligosaccharide biosynthetic process; protein amino acid glycosylation

size1 :

0.01 mg

price1 :

225 USD

size2 :

0.05 mg

price2 :

510

size3 :

0.1 mg

price3 :

795

more info or order :

MyBioSource product webpage