Recombinant Glutathione S Transferase Alpha 2 (GSTa2) | MyBioSource MBS2011062 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Glutathione S Transferase Alpha 2 (GSTa2)

catalog :

MBS2011062

quantity :

0.01 mg

price :

175 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2011062

products type :

Recombinant Protein

products full name :

Recombinant Glutathione S Transferase Alpha 2 (GSTa2)

products short name :

Glutathione S Transferase Alpha 2 (GSTa2)

other names :

glutathione S-transferase A2; Glutathione S-transferase A2; glutathione S-transferase A2; GST-gamma; liver GST2; GST HA subunit 2; GST class-alpha member 2; glutathione S-aryltransferase A2; glutathione S-alkyltransferase A2; glutathione S-aralkyltransferase A2; S-(hydroxyalkyl)glutathione lyase A2; glutathione S-transferase alpha 2; GST HA subunit 2; GST class-alpha member 2; GST-gamma; GSTA2-2; GTH2

products gene name :

GSTa2

other gene names :

GSTA2; GSTA2; GST2; GTA2; GTH2; GSTA2-2; GST2

uniprot entry name :

GSTA2_HUMAN

host :

E Coli

sequence length :

222

sequence :

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGS-MAEKPKLHYS NIRGRMESIR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKEKA LIDMYIEGIA DLGEMILLLP FSQPEEQDAK LALIQEKTKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEESRKIF RF

purity :

> 95%

form :

Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.05% sarcosyl and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Met1~Phe222 (Accession # P09210) with N-terminal His-Tag. Predicted isoelectric point: 8.5

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

215276987

ncbi acc num :

NP_000837.3

ncbi gb acc num :

NM_000846.4

uniprot acc num :

P09210

ncbi mol weight :

26.9kDa

ncbi pathways :

AhR Pathway (755436); Biological Oxidations Pathway (105698); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Drug Metabolism - Cytochrome P450 Pathway (83032); Drug Metabolism - Cytochrome P450 Pathway (427); Glutathione Conjugation Pathway (105727); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (198824); Glutathione Metabolism Pathway (343)

ncbi summary :

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation. [provided by RefSeq, Jul 2008]

uniprot summary :

GSTA2: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Alpha family. Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; Other Amino Acids Metabolism - glutathione; Transferase; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 2.5.1.18. Chromosomal Location of Human Ortholog: 6p12.1. Cellular Component: cytosol. Molecular Function: glutathione transferase activity. Biological Process: glutathione metabolic process; epithelial cell differentiation; xenobiotic metabolic process

size1 :

0.01 mg

price1 :

175 USD

size2 :

0.05 mg

price2 :

360

size3 :

0.1 mg

price3 :

560

more info or order :

MyBioSource product webpage