protein

Recombinant Polybromo 1 (PBRM1)

MBS2011038

0.01 mg

165 USD

Recombinant Protein

Recombinant Polybromo 1 (PBRM1)

[Polybromo 1 (PBRM1)]

[protein polybromo-1; Protein polybromo-1; protein polybromo-1; polybromo-1D; BRG1-associated factor 180; polybromo 1; BRG1-associated factor 180; BAF180; Polybromo-1D]

[PBRM1]

[PBRM1; PBRM1; PB1; BAF180; BAF180; PB1; hPB1; BAF180]

PB1_HUMAN

E Coli

Homo sapiens (Human)

1582

> 90%

Original Concentration: 200 Ug/mL

Storage: Avoid repeated freeze/thaw cycles . Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Positive Control, Immunogen, SDS-PAGE, WB. (May be suitable for use in other assays to be determined by the end user.)

SDS-Page

. Source: Prokaryotic expression. Residues: Met1~Ala306. Tags: N-terminal His and GST Tag. Subcellular Location: Nucleus. Traits: Freeze-dried powder. Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose and Proclin300.

. Predicted isoelectric point: 6.5. Predicted Molecular Mass: 65.0 kDa. Accurate Molecular Mass: 65kDa as determined by SDS-PAGE reducing conditions. [ USAGE ]: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

93102371

NP_060783.3

NM_018313.4

Q86U86

This locus encodes a subunit of ATP-dependent chromatin-remodeling complexes. The encoded protein has been identified as in integral component of complexes necessary for ligand-dependent transcriptional activation by nuclear hormone receptors. Mutations at this locus have been associated with primary clear cell renal cell carcinoma. [provided by RefSeq, Feb 2012]

polybromo 1: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Acts as a negative regulator of cell proliferation. Defects in PBRM1 are a cause of renal cell carcinoma (RCC). It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. 9 isoforms of the human protein are produced by alternative splicing. Protein type: Tumor suppressor; DNA-binding. Chromosomal Location of Human Ortholog: 3p21. Cellular Component: kinetochore; nuclear chromosome. Molecular Function: protein binding; DNA binding; chromatin binding. Biological Process: chromatin remodeling; negative regulation of cell proliferation; mitosis; regulation of transcription, DNA-dependent; transcription, DNA-dependent; heart development; placenta development

0.01 mg

165 USD

0.05 mg

335

0.1 mg

515

0.2 mg

635

0.5 mg

1235

1 mg

1845