Recombinant Connexin 40 (CX40) | MyBioSource MBS2010997 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Connexin 40 (CX40)

catalog :

MBS2010997

quantity :

0.01 mg

price :

135 USD

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

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product information

catalog number :

MBS2010997

products type :

Recombinant Protein

products full name :

Recombinant Connexin 40 (CX40)

products short name :

[Connexin 40 (CX40)]

other names :

[gap junction alpha-5 protein; Gap junction alpha-5 protein; gap junction alpha-5 protein; connexin 40; connexin-40; gap junction membrane channel protein alpha 5; gap junction protein, alpha 5; Connexin-40]

products gene name :

[CX40]

other gene names :

[Gja5; Gja5; Cx40; Cxn-40; Cx40]

uniprot entry name :

CXA5_RAT

host :

E Coli

reactivity :

Rat

purity :

>98%

form :

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300

storage stability :

Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for two years. Stability Test : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling

app notes :

May be suitable for use in other assays to be determined by the end user.

image1 heading :

Sequence Information

image2 heading :

SDS-Page

image3 heading :

Testing Data

other info1 :

Source: Prokaryotic expression. Original Concentration: 200ug/mL. Traits: Freeze-dried powder. Residues: Ala50~Glu303. Tags: N-terminal His-Tag. Tissue Specificity: Lung. Subcellular Location: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction

other info2 :

Predicted isoelectric point: 6.9. Predicted Molecular Mass: 30.1kDa. Accurate Molecular Mass: 20&16kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:. 1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation,glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

9506725

ncbi acc num :

NP_062153.1

ncbi gb acc num :

NM_019280.1

uniprot acc num :

P28234

ncbi pathways :

Calcium Regulation In The Cardiac Cell Pathway (198495)

ncbi summary :

forms intercellular connoxin channels in gap junctions; mediates cell-cell communication [RGD, Feb 2006]

uniprot summary :

GJA5: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Defects in GJA5 are a cause of familial atrial standstill (FAS). Atrial standstill is an extremely rare arrhythmia, characterized by the absence of electrical and mechanical activity in the atria. Electrocardiographically, it is characterized by bradycardia, the absence of P waves, and a junctional narrow complex escape rhythm. Defects in GJA5 are the cause of familial atrial fibrillation type 11 (ATFB11). ATFB11 is a familial form of atrial fibrillation, a common sustained cardiac rhythm disturbance. Atrial fibrillation is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the connexin family. Alpha-type (group II) subfamily. Protein type: Motility/polarity/chemotaxis; Channel, misc.; Membrane protein, integral; Membrane protein, multi-pass. Cellular Component: connexon complex; cell projection; integral to plasma membrane; gap junction. Molecular Function: gap junction hemi-channel activity; gap junction channel activity. Biological Process: blood vessel development; negative regulation of glomerular filtration; gap junction assembly; regulation of vasodilation; heart development; positive regulation of vasodilation; protein oligomerization; positive regulation of vasoconstriction; regulation of systemic arterial blood pressure; regulation of systemic arterial blood pressure by renal system process; artery morphogenesis; embryonic heart tube development; negative regulation of blood pressure; angiogenesis; transmembrane transport; skeletal development; potassium ion transport; embryonic limb morphogenesis

size1 :

0.01 mg

price1 :

135 USD

size2 :

0.05 mg

price2 :

250

size3 :

0.1 mg

price3 :

385

size4 :

0.2 mg

price4 :

470

size5 :

0.5 mg

price5 :

910

size6 :

1 mg

price6 :

1345

more info or order :

MyBioSource product webpage