protein

Recombinant Superoxide Dismutase 3, Extracellular (SOD3)

MBS2010936

0.01 mg

160 USD

Recombinant Protein

Recombinant Superoxide Dismutase 3, Extracellular (SOD3)

[Superoxide Dismutase 3, Extracellular (SOD3)]

[extracellular superoxide dismutase; Extracellular superoxide dismutase [Cu-Zn]; extracellular superoxide dismutase [Cu-Zn]; extracellular superoxide dismutase [Cu-Zn]; superoxide dismutase 3, extracellular]

[SOD3]

[Sod3; Sod3; EC-SOD; AI314465; EC-SOD]

SODE_MOUSE

E Coli

>92%

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degreeC for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-PAGE, WB, ELISA, IP, CoIP, Purification, Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

Sequence Information

SDS-Page

Source: Prokaryotic expression. Residues: Ser25~Thr251. Tags: N-terminal His-Tag. Tissue Specificity: Lung, Kidney, Liver. Subcellular Location: Secreted, extracellular space. Traits: Freeze-dried powder

Predicted isoelectric point: 6.8. Predicted Molecular Mass: 26.2kDa. Accurate Molecular Mass: 31&33kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation: . The possible reasons that the actual band size differs from the predicted are as follows:. 1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.5. Polymerization of the target protein: Dimerization, multimerization etc. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

6755600

NP_035565.1

NM_011435.3

O09164

Cellular Responses To Stress Pathway (925999); Detoxification Of Reactive Oxygen Species Pathway (926005); One Carbon Metabolism And Related Pathways (198380); Oxidative Stress Pathway (198384); Superoxide Radicals Degradation Pathway (143050)

SOD3: Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. Belongs to the Cu-Zn superoxide dismutase family. Protein type: EC 1.15.1.1; Secreted; Secreted, signal peptide; Oxidoreductase. Cellular Component: extracellular matrix; extracellular space; cytoplasm; extracellular region; trans-Golgi network; nucleus. Molecular Function: antioxidant activity; protein binding; copper ion binding; zinc ion binding; metal ion binding; superoxide dismutase activity; oxidoreductase activity. Biological Process: response to copper ion; removal of superoxide radicals; response to hypoxia; superoxide metabolic process

0.01 mg

160 USD

0.05 mg

315

0.1 mg

490

0.2 mg

600

0.5 mg

1170

1 mg

1745