Recombinant Major Basic Protein (MBP) | MyBioSource MBS2010924 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Major Basic Protein (MBP)

catalog :

MBS2010924

quantity :

0.01 mg

price :

190 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2010924

products type :

Recombinant Protein

products full name :

Recombinant Major Basic Protein (MBP)

products short name :

Major Basic Protein (MBP)

other names :

bone marrow proteoglycan isoform 2 preproprotein; Bone marrow proteoglycan; bone marrow proteoglycan; bone-marrow proteoglycan; proteoglycan 2 preproprotein; natural killer cell activator; eosinophil major basic protein; eosinophil granule major basic protein; proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein); Proteoglycan 2Cleaved into the following chain:Eosinophil granule major basic protein; EMBP; MBP; Alternative name(s):; Pregnancy-associated major basic protein

products gene name :

MBP

other gene names :

PRG2; PRG2; MBP; BMPG; MBP1; MBP; BMPG; EMBP; MBP

uniprot entry name :

PRG2_HUMAN

host :

E Coli

sequence length :

211

sequence :

The sequence of the target protein is listed below. QTCRYL LVRSLQTFSQ AWFTCRRCYR GNLVSIHNFN INYRIQCSVS ALNQGQVWIG GRITGSGRCR RFQWVDGSRW NFAYWAAHQP WSRGGHCVAL CTRGGHWRRA HCLRRLPFIC SY

purity :

> 90%

form :

Supplied as lyophilized form in 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Gln105~Tyr222 (Accession # P13727) with two N-terminal Tags, His-tag and MBP-tag

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile ddH2O. Subcellular Location: Cytoplasmic vesicle, secretory vesicle. Predicted isoelectric point: 8.4. Predicted Molecular Mass: 60.5kDa. Accurate Molecular Mass: 64kDa as determined by SDS-PAGE reducing conditions.

ncbi gi num :

342837699

ncbi acc num :

NP_001230174.1

ncbi gb acc num :

NM_001243245.1

uniprot acc num :

P13727

ncbi pathways :

Asthma Pathway (83120); Asthma Pathway (532)

ncbi summary :

The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. The encoded protein contains a peptide that displays potent antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria, and fungi. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

uniprot summary :

PRG2: Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA. Protein type: Secreted, signal peptide; Secreted; Vesicle. Chromosomal Location of Human Ortholog: 11q12. Cellular Component: transport vesicle; extracellular region. Molecular Function: heparin binding; carbohydrate binding. Biological Process: positive regulation of interleukin-4 production; negative regulation of interleukin-10 production; defense response to bacterium; immune response; defense response to nematode

size1 :

0.01 mg

price1 :

190 USD

size2 :

0.05 mg

price2 :

395

size3 :

0.1 mg

price3 :

610

more info or order :

MyBioSource product webpage