catalog number :
MBS2010896
products type :
Recombinant Protein
products full name :
Recombinant A Disintegrin And Metalloprotease 5 (ADAM5)
products short name :
A Disintegrin And Metalloprotease 5 (ADAM5)
other names :
disintegrin and metalloproteinase domain-containing protein 5; Disintegrin and metalloproteinase domain-containing protein 5; disintegrin and metalloproteinase domain-containing protein 5; tMDC II; ADAM metallopeptidase domain 5; a disintegrin and metalloprotease domain 5; a disintegrin and metalloprotease domain (ADAM) 5; transmembrane metalloproteinase-like, disintegrin-like, and cysteine-rich protein II; a disintegrin and metallopeptidase domain 5; Transmembrane metalloproteinase-like, disintegrin-like, and cysteine-rich protein II
products gene name :
ADAM5
other gene names :
Adam5; Adam5; tMDCII; Tmdc2; tMDC II
uniprot entry name :
ADAM5_RAT
sequence :
The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-CDFEEYCN GNDAYCVPDT FARNGQYCDS GQAFCYSGLC MTSNNQCMNL LGKYVRGASF ACYEEFNSRN DRFGNCIRKF CSFENSLCGK LVCTWPFKRL LMKDNMSAAY GQIRDDLCIS LYKGGRPLKT TLSTYSDMSE RDETFVKDGT ICGPDMFCLE TQCKETRFLV DFQQCNTSRD CNDHGVCNNF NHCHCDKGYN PPYCESVKGQ FGSIDDGHKY YIDEGKSAKQ QNRGIHPKQ
form :
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
storage stability :
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
other info1 :
Organism: Rattus norvegicus (Rat). Expression System: Prokaryotic expression. Residues: Cys413~Gln649 (Accession # Q5BK84) with N-terminal His-Tag
other info2 :
Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
products description :
About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.
ncbi acc num :
NP_064699.1
ncbi gb acc num :
NM_020303.1
ncbi mol weight :
28.4kDa
ncbi summary :
germ-cell specific metalloprotease-disintegrin that may play a role in cell-cell and cell-matrix interactions during spermatogenesis [RGD, Feb 2006]
uniprot summary :
ADAM5P: This is a non catalytic metalloprotease-like protein. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Protease. Cellular Component: integral to membrane. Molecular Function: zinc ion binding; metalloendopeptidase activity. Biological Process: proteolysis