protein

Recombinant Klotho (KL)

MBS2010881

0.01 mg

135 USD

Recombinant Protein

Recombinant Klotho (KL)

[Klotho (KL)]

[klotho; Klotho; klotho; secreted form of Klotho protein; klotho]

[KL]

[Kl; Kl; alpha-kl]

KLOT_MOUSE

E.coli

> 97%

Original Concentration: 200 Ug/mL

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

Sequence Information

SDS-Page

Organism Species: Mus musculus (Mouse). Source: Prokaryotic expression. Residues: Pro516~Phe955. Tags: N-terminal His Tag. Subcellular Location: Membrane, Secreted. Traits: Freeze-dried powder. Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Predicted isoelectric point: 6.4. Predicted Molecular Mass: 52.3kDa. Accurate Molecular Mass: 52kDa as determined by SDS-PAGE reducing conditions. USAGE: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

227330584

NP_038851.2

NM_013823.2

O35082

Adaptive Immune System Pathway (926013); Ascorbate Biosynthesis, Animals, Glucose-1P = Ascorbate Pathway (421792); Ascorbate Biosynthesis, Animals, Glucose-1P = Ascorbate Pathway (468309); Constitutive PI3K/AKT Signaling In Cancer Pathway (926316); DAP12 Interactions Pathway (926127); DAP12 Signaling Pathway (926128); Disease Pathway (926229); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (926027); Downstream Signal Transduction Pathway (926957); Downstream Signaling Of Activated FGFR Pathway (926257)

Klotho: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Hydrolase; EC 3.2.1.31; Membrane protein, integral. Cellular Component: extracellular space; membrane; extracellular region; plasma membrane; integral to membrane. Molecular Function: protein binding; signal transducer activity; beta-glucuronidase activity; fibroblast growth factor binding; hydrolase activity; hydrolase activity, acting on glycosyl bonds; hydrolase activity, hydrolyzing O-glycosyl compounds; fibroblast growth factor receptor binding. Biological Process: calcium ion homeostasis; fibroblast growth factor receptor signaling pathway; metabolic process; carbohydrate metabolic process; energy reserve metabolic process; insulin receptor signaling pathway; positive regulation of bone mineralization; acute inflammatory response; aging

0.01 mg

135 USD

0.05 mg

245

0.1 mg

365

0.2 mg

450

0.5 mg

870

1 mg

1280