Recombinant Phospholipase A2, Lipoprotein Associated (LpPLA2) | MyBioSource MBS2010829 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Phospholipase A2, Lipoprotein Associated (LpPLA2)

catalog :

MBS2010829

quantity :

0.01 mg

price :

175 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2010829

products type :

Recombinant Protein

products full name :

Recombinant Phospholipase A2, Lipoprotein Associated (LpPLA2)

products short name :

Phospholipase A2, Lipoprotein Associated (LpPLA2)

other names :

platelet-activating factor acetylhydrolase; Platelet-activating factor acetylhydrolase; platelet-activating factor acetylhydrolase; LDL-PLA(2); gVIIA-PLA2; PAF 2-acylhydrolase; PAF acetylhydrolase; group-VIIA phospholipase A2; LDL-associated phospholipase A2; lipoprotein-associated phospholipase A2; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma); 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; Group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA(2); PAF 2-acylhydrolase

products gene name :

LpPLA2

other gene names :

PLA2G7; PLA2G7; PAFAD; PAFAH; LP-PLA2; LDL-PLA2; PAFAH; PAF acetylhydrolase; gVIIA-PLA2; LDL-PLA(2)

uniprot entry name :

PAFA_HUMAN

host :

E Coli

sequence length :

441

sequence :

The sequence of the target protein is listed below. FDWQYINPV AHMKSSAWVN KIQVLMAAAS FGQTKIPRGN GPYSVGCTDL MFDHTNKGTF LRLYYPSQDN DRLDTLWIPN KEYFWGLSKF LGTHWLMGNI LRLLFGSMTT PANWNSPLRP GEKYPLVVFS HGLGAFRTLY SAIGIDLASH GFIVAAVEHR DRSASATYYF KDQSAAEIGD KSWLYLRTLK QEEETHIRNE QVRQRAKECS QALSLILDID HGKPVKNALD LKFDMEQLKD SIDREKIAVI GHSFGGATVI QTLSEDQRFR CGIALDAWMF PLGDEVYSRI PQPLFFINSE YFQYPANIIK MKKCYSPDKE RKMITIRGSV HQNFADFTFA TGKIIGHMLK LKGDIDSNVA IDLSNKASLA FLQKHLGLHK DFDQWDCLIE GDDENLIPGT NINTTNQHIM LQNSSGIEKY N

purity :

>95%

form :

Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.05% sarcosyl and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Phe22~Asn441 (Accession # Q13093) with N-terminal His-Tag. Subcellular Location: Secreted, extracellular space

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Predicted Isoelectric Point: 7.1. Reconstitution: Reconstitute in sterile ddH2O.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

270133071

ncbi acc num :

NP_001161829.1

ncbi gb acc num :

NM_001168357.1

uniprot acc num :

Q13093

ncbi mol weight :

50.0kDa

ncbi pathways :

Ether Lipid Metabolism Pathway (82990); Ether Lipid Metabolism Pathway (365); Lissencephaly Gene (LIS1) In Neuronal Migration And Development Pathway (137984); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Synthesis, Secretion, And Deacylation Of Ghrelin Pathway (119538)

ncbi summary :

The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2009]

uniprot summary :

PLA2G7: Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PAFAD). An enzymatic deficiency that results in exacerbated bodily response to inflammatory agents. Asthmatic individuals affected by this condition may manifest severe respiratory symptoms. Defects in PLA2G7 are a cause of susceptibility to asthma (ASTHMA). The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi. PLA2G7 variants can be a risk factor for the development of asthma and PLA2G7 may act as a modifier gene that modulates the severity of this disease. Defects in PLA2G7 are a cause of susceptibility to atopic hypersensitivity (ATOPY). A condition characterized by predisposition to develop hypersensitivity reactions. Atopic individuals can develop eczema, allergic rhinitis and allergic asthma. Belongs to the AB hydrolase superfamily. Lipase family. Protein type: EC 3.1.1.47; Hydrolase; Secreted; Lipid Metabolism - ether lipid; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 6p21.2-p12. Cellular Component: cytoplasm; extracellular region. Molecular Function: calcium-independent phospholipase A2 activity; phospholipid binding; 1-alkyl-2-acetylglycerophosphocholine esterase activity. Biological Process: cellular protein metabolic process; lipid catabolic process; positive regulation of inflammatory response. Disease: Asthma, Susceptibility To; Ige Responsiveness, Atopic; Platelet-activating Factor Acetylhydrolase Deficiency

size1 :

0.01 mg

price1 :

175 USD

size2 :

0.05 mg

price2 :

360

size3 :

0.1 mg

price3 :

560

more info or order :

MyBioSource product webpage