Recombinant Low Density Lipoprotein Receptor Related Protein 6 (LRP6) | MyBioSource MBS2010787 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Low Density Lipoprotein Receptor Related Protein 6 (LRP6)

catalog :

MBS2010787

quantity :

0.01 mg

price :

140 USD

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

product information

catalog number :

MBS2010787

products type :

Recombinant Protein

products full name :

Recombinant Low Density Lipoprotein Receptor Related Protein 6 (LRP6)

products short name :

[Low Density Lipoprotein Receptor Related Protein 6 (LRP6)]

other names :

[low-density lipoprotein receptor-related protein 6; Low-density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LRP-6; low density lipoprotein receptor-related protein 6]

products gene name :

[LRP6]

other gene names :

[LRP6; LRP6; ADCAD2; LRP-6]

uniprot entry name :

LRP6_HUMAN

host :

E. coli

reactivity :

Homo sapiens (Human)

sequence length :

1613

sequence :

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-WIDSRQNMIR KAQEDGSQGF TVVVSSVPSQ NLEIQPYDLS IDIYSRYIYW TCEATNVINV TRLDGRSVGV VLKGEQDRPR AVVVNPEKGY MYFTNLQERS PKIERAALDG TEREVLFFSG LSKPIALALD SRLGKLFWAD SDLRRIESSD LSGANRIVLE DSNILQPVGL TVFENWLYWI DKQQQMIEKI DMTGREGRTK VQARIAQLSD IHAVKELNLQ EYRQHPCAQD NGG

purity :

>92%

form :

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

Positive Control, Immunogen, SDS-PAGE, WB. (May be suitable for use in other assays to be determined by the end user.)

image1 heading :

Sequence Information

image2 heading :

SDS-Page

other info1 :

Source: Prokaryotic expression. Residues: Trp981~Gly1213. Tags: N-terminal His-Tag. Tissue Specificity: Kidney, Brain. Subcellular Location: Membrane; Single-pass type I membrane protein. Endoplasmic reticulum. Traits: Freeze-dried powder

other info2 :

Predicted isoelectric point: 6.3. Predicted Molecular Mass: 30.3kDa. Accurate Molecular Mass: 30kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

148727288

ncbi acc num :

NP_002327.2

ncbi gb acc num :

NM_002336.2

uniprot acc num :

O75581

ncbi pathways :

Canonical Wnt Signaling Pathway (138032); MicroRNAs In Cardiomyocyte Hypertrophy Pathway (198784); Presenilin Action In Notch And Wnt Signaling Pathway (137914); Wnt Signaling Pathway NetPath (198799); Wnt Signaling Pathway And Pluripotency (198847); Wnt Signaling Network Pathway (137962); Wnt Signaling Pathway (83061); Wnt Signaling Pathway (471)

ncbi summary :

This gene encodes a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor-mediated endocytosis of lipoprotein and protein ligands. The protein encoded by this gene functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. This protein undergoes gamma-secretase dependent RIP- (regulated intramembrane proteolysis) processing but the precise locations of the cleavage sites have not been determined.[provided by RefSeq, Dec 2009]

uniprot summary :

LRP6: Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor- ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3- mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation. Homodimer; disulfide-linked. Forms phosphorylated oligomer aggregates on Wnt-signaling. Forms a WNT-signaling complex formed of a WNT protein, a FZD protein and LRP5 or LRP6. Interacts (via the extracellular domain) with WNT1; the interaction is enhanced by prior formation of the Wnt/Fzd complex. Interacts (via the beta-propeller regions 3 and 4) with WNT3A. Interacts (via the beta-propeller regions 1 and 2) with WNT9B. Interacts with FZD5; the interaction forms a coreceptor complex for Wnt signaling and is inhibited by DKK1 and C1orf187. Interacts (via beta propeller region) with DKK1; the interaction inhibits FZD5/LRP6 complex formation. Interacts with DKK2. Interacts with C1orf187/DRAXIN; the interaction inhibits Wnt signaling. Interacts (via the phosphorylated PPPSP motifs) with AXIN1; the interaction recruits the AXIN1/GSK3B complex to cell surface LRP6 signalsomes. Interacts with GRB10; the interaction prevents AXIN1 binding, thus negatively regulating the Wnt signaling pathway. Interacts (via the extracellular domain) with RSPO1; the interaction activates Wnt/beta-catenin signaling. Interacts (via the extracellular domain) with RSPO3 (via the cysteine rich domain); the interaction activates Wnt/beta-catenin signaling. Interacts (via the beta- propeller regions 1 and 2) with SOST; the interaction competes with DKK1 for binding for inhibiting beta-catenin signaling. Interacts with MESD; the interaction prevents the formation of LRP6 aggregates and targets LRP6 to the plasma membrane. Interacts (via the cytoplasmic domain) with CSNKIE; the interaction phosphorylates LRP6, binds AXIN1 and inhibits AXIN1/GSK3B-mediated phosphorylation of beta-catenin. Interacts with MACF1. Decreased levels on WNT3A stimulation. Widely co-expressed with LRP5 during embryogenesis and in adult tissues. Belongs to the LDLR family. Protein type: Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 12p13.2. Cellular Component: Golgi apparatus; cell surface; endoplasmic reticulum; early endosome; extracellular region; integral to membrane; caveola; cell soma; early endosome membrane; plasma membrane; synapse; cytoplasmic vesicle; receptor complex. Molecular Function: low-density lipoprotein receptor activity; Wnt-protein binding; identical protein binding; Wnt receptor activity; protein binding; protein homodimerization activity; frizzled binding; kinase inhibitor activity; toxin transporter activity; apolipoprotein binding; receptor binding. Biological Process: radial glial cell differentiation in the forebrain; positive regulation of apoptosis; regulation of fat cell differentiation; positive regulation of transcription, DNA-dependent; cerebellum morphogenesis; Wnt receptor signaling pathway through beta-catenin; bone remodeling; embryonic pattern specification; Wnt receptor signaling pathway in forebrain neuroblast division; anterior/posterior pattern formation; elevation of cytosolic calcium ion concentration; cell-cell adhesion; convergent extension; response to folic acid; positive regulation of mesenchymal cell proliferation; midbrain development; embryonic limb morphogenesis; external genitalia morphogenesis; neural crest formation; positive regulation of cell cycle; negative regulation of fat cell differentiation; midbrain-hindbrain boundary development; negative regulation of protein kinase activity; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development; embryonic digit morphogenesis; regulation of ossification; embryonic forelimb morphogenesis; response to peptide hormone stimulus; palate development; thalamus development; embryonic hindlimb morphogenesis; synaptic transmission; negative regulation of protein amino acid phosphorylation; positive regulation of bone resorption; heart looping; neural crest cell differentiation; cell migration involved in gastrulation; negative regulation of epithelial cell proliferation; Wnt receptor signaling pathway; gastrulation with mouth forming second; positive regulation of ossification; odontogenesis of dentine-containing teeth; formation of radial glial scaffolds; cerebral cortex cell migration; embryonic retina morphogenesis in camera-type eye; neural tube closure; embryonic camera-type eye morphogenesis. Disease: Coronary Artery Disease, Autosomal Dominant 2

size1 :

0.01 mg

price1 :

140 USD

size2 :

0.05 mg

price2 :

270

size3 :

0.1 mg

price3 :

420

size4 :

0.2 mg

price4 :

515

size5 :

0.5 mg

price5 :

990

size6 :

1 mg

price6 :

1475

more info or order :

MyBioSource product webpage