Recombinant Parathyroid Hormone (PTH) | MyBioSource MBS2010769 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Parathyroid Hormone (PTH)

catalog :

MBS2010769

quantity :

0.01 mg

price :

185 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2010769

products type :

Recombinant Protein

products full name :

Recombinant Parathyroid Hormone (PTH)

products short name :

Parathyroid Hormone (PTH)

other names :

parathyroid hormone; Parathyroid hormone; parathyroid hormone; parathyroid hormone

products gene name :

PTH

other gene names :

Pth; Pth; Pthp

uniprot entry name :

Q9Z0L6_MOUSE

host :

E Coli

sequence length :

115

sequence :

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below. MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- KPVRK RAVSEIQLMH NLGKHLASME RMQWLRRKLQ DMHNFVSLGV QMAARDGSHQ KPTKKEENVL VDGNPKSLGE GDKADVDVLV KSKSQ

purity :

> 95%

form :

Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Mus musculus (Mouse). Expression System: Prokaryotic expression. Residues: Lys26~Gln115 (Accession # Q9Z0L6) with two N-terminal Tags, His-tag and GST-tag. Predicted isoelectric point: 7.3

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

10181174

ncbi acc num :

NP_065648.1

ncbi gb acc num :

NM_020623.2

uniprot acc num :

Q9Z0L6

ncbi mol weight :

42.2kDa

ncbi pathways :

Class B/2 (Secretin Family Receptors) Pathway (927016); Endochondral Ossification Pathway (198336); G Alpha (s) Signalling Events Pathway (927021); GPCR Downstream Signaling Pathway (927020); GPCR Ligand Binding Pathway (926990); Osteoblast Pathway (198413); Signal Transduction Pathway (926897); Signaling By GPCR Pathway (926989)

uniprot summary :

PTH: PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2- deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells. Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH); also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism. Belongs to the parathyroid hormone family. Protein type: Secreted; Secreted, signal peptide; Hormone; Apoptosis. Cellular Component: extracellular space; cell; intracellular. Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; type 1 parathyroid hormone receptor binding; parathyroid hormone receptor binding; peptide hormone receptor binding; hormone activity. Biological Process: transcription from RNA polymerase II promoter; positive regulation of signal transduction; positive regulation of glycogen biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; positive regulation of bone mineralization; G-protein signaling, adenylate cyclase activating pathway; Rho protein signal transduction; positive regulation of ossification; cellular calcium ion homeostasis; positive regulation of glucose import; cell-cell signaling; regulation of gene expression; positive regulation of cAMP biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; skeletal development; bone resorption. Disease: Hypoparathyroidism, Familial Isolated

size1 :

0.01 mg

price1 :

185 USD

size2 :

0.05 mg

price2 :

395

size3 :

0.1 mg

price3 :

605

more info or order :

MyBioSource product webpage