catalog number :
MBS2010659
products type :
Recombinant Protein
products full name :
Recombinant Mercaptopyruvate Sulfurtransferase (MPST)
products short name :
Mercaptopyruvate Sulfurtransferase (MPST)
other names :
3-mercaptopyruvate sulfurtransferase isoform 2; 3-mercaptopyruvate sulfurtransferase; 3-mercaptopyruvate sulfurtransferase; human liver rhodanese; mercaptopyruvate sulfurtransferase
products gene name :
MPST
other gene names :
MPST; MPST; MST; TST2; TST2; MST
uniprot entry name :
THTM_HUMAN
sequence :
The sequence of the target protein is listed below. RTS PYDHMLPGAE HFAEYAGRLG VGAATHVVIY DASDQGLYSA PRVWWMFRAF GHHAVSLLDG GLRHWLRQNL PLSSGKSQPA PAEFRAQLDP AFIKTYEDIK ENLESRRFQV VDSRATGRFR GTEPEPRDGI EPGHIPGTVN IPFTDFLSQE GLEKSPEEIR HLFQEKKVDL SKPLVATCGS GVTACHVALG AYLCGKPDVP IYDGSWVE
form :
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
storage stability :
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
other info1 :
Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Arg68~Glu278 (Accession # P25325) with two N-terminal Tags, His-tag and T7-tag. Subcellular Location: Cytoplasm. Mitochondrion. Cell junction, synapse, synaptosome
other info2 :
Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Predicted Isoelectric Point: 6.5. Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
products description :
About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.
ncbi acc num :
NP_001013454.1
ncbi gb acc num :
NM_001013436.2
ncbi mol weight :
52.6kDa
ncbi pathways :
Cysteine And Methionine Metabolism Pathway (104488); Cysteine And Methionine Metabolism Pathway (103421); Sulfur Metabolism Pathway (83026); Sulfur Metabolism Pathway (417); Sulfur Relay System Pathway (172845); Sulfur Relay System Pathway (171866); Trans-sulfuration Pathway (739008)
uniprot summary :
MPST: Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. May have a role in cyanide degradation or in thiosulfate biosynthesis. Protein type: Amino Acid Metabolism - cysteine and methionine; EC 2.8.1.2; Mitochondrial; Transferase. Chromosomal Location of Human Ortholog: 22q13.1. Cellular Component: neuron projection; mitochondrial inner membrane; synapse; cell junction. Molecular Function: 3-mercaptopyruvate sulfurtransferase activity; thiosulfate sulfurtransferase activity. Biological Process: cyanate catabolic process; response to toxin