Recombinant Collagen Type I Alpha 1 (COL1a1) | MyBioSource MBS2010603 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Collagen Type I Alpha 1 (COL1a1)

catalog :

MBS2010603

quantity :

0.01 mg

price :

135 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2010603

products type :

Recombinant Protein

products full name :

Recombinant Collagen Type I Alpha 1 (COL1a1)

products short name :

[Collagen Type I Alpha 1 (COL1a1)]

other names :

[collagen alpha-1(I) chain; Collagen alpha-1(I) chain; collagen alpha-1(I) chain; collagen alpha-1(I) chain; alpha-1 type 1 collagen; alpha-1 type I collagen; procollagen, type I, alpha 1; collagen, type I, alpha 1; Alpha-1 type I collagen]

products gene name :

[COL1a1]

other gene names :

[Col1a1; Col1a1; Cola1; Mov13; Cola-1; Mov-13; Col1a-1; Cola1]

uniprot entry name :

CO1A1_MOUSE

host :

E Coli

purity :

> 95%

concentration :

Original Concentration: 200 Ug/mL

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

image1 heading :

SDS-Page

other info1 :

Organism Species: Mus musculus (Mouse). Source: Prokaryotic expression. Residues: Lys1225~Val1453. Tags: N-terminal His Tag. Subcellular Location: Secreted, Extracellular matrix. Traits: Freeze-dried powder. Buffer formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

other info2 :

Predicted isoelectric point: 6.2. Predicted Molecular Mass: 26.8kDa. Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:. 1.Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. USAGE: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

34328108

ncbi acc num :

NP_031768.2

ncbi gb acc num :

NM_007742.3

uniprot acc num :

P11087

ncbi pathways :

Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (927064); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (926223); Collagen Biosynthesis And Modifying Enzymes Pathway (927063); Collagen Formation Pathway (927062); ECM-receptor Interaction Pathway (83265); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (927061); Focal Adhesion Pathway (198353)

ncbi summary :

This gene encodes the pro-alpha1 chains of type I collagen, whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis, and tendon. [provided by RefSeq, Sep 2015]

uniprot summary :

COL1A1: Type I collagen is a member of group I collagen (fibrillar forming collagen). Defects in COL1A1 are the cause of Caffey disease (CAFFD); also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age. Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1); also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome. Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A); also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations. Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1). A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta. Defects in COL1A1 are a cause of osteogenesis imperfecta type 2 (OI2); also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency. Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3). A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta. Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4); also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta. Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP); also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture. A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF. Belongs to the fibrillar collagen family. Protein type: Secreted, signal peptide; Secreted; Extracellular matrix. Cellular Component: Golgi apparatus; extracellular matrix; proteinaceous extracellular matrix; extracellular space; collagen; endoplasmic reticulum; cytoplasm; extracellular region; collagen type I; secretory granule. Molecular Function: identical protein binding; protein binding; extracellular matrix structural constituent; platelet-derived growth factor binding; metal ion binding. Biological Process: skin development; blood vessel development; intramembranous ossification; collagen fibril organization; skin morphogenesis; wound healing; embryonic skeletal development; positive regulation of transcription, DNA-dependent; protein transport; sensory perception of sound; visual perception; skeletal morphogenesis; skeletal development; collagen biosynthetic process; positive regulation of cell migration; endochondral ossification

size1 :

0.01 mg

price1 :

135 USD

size2 :

0.05 mg

price2 :

250

size3 :

0.1 mg

price3 :

375

size4 :

0.2 mg

price4 :

460

size5 :

0.5 mg

price5 :

890

size6 :

1 mg

price6 :

1315

more info or order :

MyBioSource product webpage