Recombinant Alpha-Fodrin (SPTAN1) | MyBioSource MBS2010596 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Alpha-Fodrin (SPTAN1)

catalog :

MBS2010596

quantity :

0.01 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2010596

products type :

Recombinant Protein

products full name :

Recombinant Alpha-Fodrin (SPTAN1)

products short name :

Alpha-Fodrin (SPTAN1)

other names :

spectrin alpha chain, non-erythrocytic 1 isoform 1; Spectrin alpha chain, non-erythrocytic 1; spectrin alpha chain, non-erythrocytic 1; alpha-fodrin; alpha-II spectrin; fodrin alpha chain; spectrin, non-erythroid alpha chain; spectrin, non-erythroid alpha subunit; spectrin, alpha, non-erythrocytic 1; Alpha-II spectrin; Fodrin alpha chain; Spectrin, non-erythroid alpha subunit

products gene name :

SPTAN1

other gene names :

SPTAN1; SPTAN1; NEAS; EIEE5; SPTA2; NEAS; SPTA2

uniprot entry name :

SPTN1_HUMAN

host :

E Coli

sequence length :

2477

sequence :

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-TASDESYKDP TNIQLSKLLS KHQKHQAFEA ELHANADRIR GVIDMGNSLI ERGACAGSED AVKARLAALA DQWQFLVQKS AEKSQKLKEA NKQQNFNTGI KDFDFWLSEV EALLASEDYG KDLASVNNLL KKHQLLEADI SAHEDRLKDL NSQADSLMTS SAFDTSQVKD

purity :

> 95%

form :

Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Homo sapiens (Human). Expression System: Prokaryotic expression. Residues: Thr1573~Asp1742 (Accession # Q13813) with N-terminal His-Tag. Subcellular Location: Cytoskeleton. Cytoplasm, cell cortex. Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative. Predicted isoelectric point: 5.7

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in ddH2O.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

194595509

ncbi acc num :

NP_001123910.1

ncbi gb acc num :

NM_001130438.2

uniprot acc num :

Q13813

ncbi mol weight :

20.4kDa

ncbi pathways :

Apoptosis Pathway (105648); Apoptotic Cleavage Of Cellular Proteins Pathway (105678); Apoptotic Execution Phase Pathway (105677); Axon Guidance Pathway (926383); Axon Guidance Pathway (105688); Caspase Cascade In Apoptosis Pathway (137974); Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway (105679); Cell-Cell Communication Pathway (477132); Developmental Biology Pathway (926382); Developmental Biology Pathway (477129)

ncbi summary :

Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

uniprot summary :

SPTAN1: a cytoskeletal protein which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. Capable to forming dimers which can further associate to tetramers. Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 9q34.11. Cellular Component: spectrin; microtubule cytoskeleton; intracellular membrane-bound organelle; membrane; fascia adherens; cytosol; Z disc; vesicle; lateral plasma membrane. Molecular Function: calmodulin binding; protein binding; spectrin binding; structural constituent of cytoskeleton; protein heterodimerization activity; calcium ion binding; actin binding. Biological Process: axon guidance; apoptosis; actin filament capping; cell structure disassembly during apoptosis. Disease: Epileptic Encephalopathy, Early Infantile, 5

size1 :

0.01 mg

price1 :

140 USD

size2 :

0.05 mg

price2 :

260

size3 :

0.1 mg

price3 :

400

more info or order :

MyBioSource product webpage