Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 2 (ADAMTS2) | MyBioSource MBS2010506 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 2 (ADAMTS2)

catalog :

MBS2010506

quantity :

0.01 mg

price :

175 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2010506

products type :

Recombinant Protein

products full name :

Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 2 (ADAMTS2)

products short name :

A Disintegrin And Metalloproteinase With Thrombospondin 2 (ADAMTS2)

other names :

A disintegrin and metalloproteinase with thrombospondin motifs 2; A disintegrin and metalloproteinase with thrombospondin motifs 2; A disintegrin and metalloproteinase with thrombospondin motifs 2; pNPI; PC I-NP; ADAM-TS2; ADAMTS-2; ADAM-TS 2; procollagen I N-proteinase; procollagen N-endopeptidase; procollagen I/II amino propeptide-processing enzyme; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 2; ADAM metallopeptidase with thrombospondin type 1 motif, 2<; Procollagen I N-proteinase; PC I-NP; Procollagen I/II amino propeptide-processing enzyme; Procollagen N-endopeptidase

products gene name :

ADAMTS2

other gene names :

ADAMTS2; ADAMTS2; NPI; ADAM-TS 2; ADAM-TS2; ADAMTS-2; PC I-NP; pNPI

uniprot entry name :

ATS2_BOVIN

host :

E Coli

sequence length :

1205

sequence :

The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGS-QLPGLHY SMNEQCRFDF GLGYMMCTAF RTFDPCKQLW CSHPDNPYFC KTKKGPPLDG TMCAPGKHCF KGHCIWLTPD ILKRDGNWGA WSPFGSCSRT CGTGVKFRTR QCDNPHPANG GRTCSGLAYD FQLCNSQDCP

purity :

> 95%

form :

Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

storage stability :

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

other info1 :

Organism: Bos taurus; Bovine (Cattle). Expression System: Prokaryotic expression. Residues: Gln474~Pro610 (Accession # P79331) with N-terminal His-Tag

other info2 :

Endotoxin Level: <1.0EU per 1ug (determined by the LAL method). Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

products description :

About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

ncbi gi num :

27807141

ncbi acc num :

NP_777056.1

ncbi gb acc num :

NM_174631.2

uniprot acc num :

P79331

ncbi mol weight :

16.6kDa

ncbi pathways :

Collagen Biosynthesis And Modifying Enzymes Pathway (934102); Collagen Formation Pathway (934101); Extracellular Matrix Organization Pathway (934100)

uniprot summary :

Function: Cleaves the propeptides of type I and II collagen prior to fibril assembly. Does not act on type III collagen. May also play a role in development that is independent of its role in collagen biosynthesis. Catalytic activity: Cleaves the N-propeptide of collagen chain alpha-1(I) at Pro- -Gln and of alpha-1(II) and alpha-2(I) at Ala- -Gln. Cofactor: Binds 1 zinc ion per subunit . By similarity. Subunit structure: May belong to a multimeric complex. Binds specifically to collagen type XIV. Subcellular location: Secreted extracellular space extracellular matrix . By similarity. Tissue specificity: Enzymatic activity is detected at high level in all type I collagen-rich tissues such as skin, bones, tendons and aorta and at low level in brain and thymus. The mRNA levels were disproportionately high in heart, liver, retina and muscle. Domain: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix. Post-translational modification: The N-terminus is blocked.The precursor is cleaved by a furin endopeptidase . By similarity.Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion . By similarity. Involvement in disease: Defects in ADAMTS2 are the cause of dermatosparaxis, a recessively inherited disorder characterized by severe skin fragility and biochemically by the presence in skin of procollagen incompletely processed at the N-terminus. Sequence similarities: Contains 1 disintegrin domain.Contains 1 peptidase M12B domain.Contains 1 PLAC domain.Contains 4 TSP type-1 domains. Caution: Has sometimes been referred to as ADAMTS3.

size1 :

0.01 mg

price1 :

175 USD

size2 :

0.05 mg

price2 :

360

size3 :

0.1 mg

price3 :

560

more info or order :

MyBioSource product webpage